Detailed Locus Information for CNAG_03024T1
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | AGC protein kinase | ||||||||||||||||||
| Gene Symbol | RIM15 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNC00600 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MSNGVFFLDEERERDTLSDPSPTLSPSNPTEDAKSTQPLPSLQVPQTTLAAPIPTLGGQPLPQHRKHPAPFAATPSFPSPLARAVTVPSHSDESSSSTHSSPTQSDEEWEGRRNSGQRSPSEMSGSGTPRRWAEGVHESHQRSPSLSRAGSPTSRPPSPTTAGTNTRPQPLTPGALLMRSRRSGSTSRHSPPRIAKKEPTTGQSMPRFVDTFSRVQTGDISSVHSQSGSSGSGHDQSSPSKSESVSDGRPVAIPSAQDSSKEADSLGLGWGTWESSGSGSSFGSGAGNLRDKGKSRGDLRDIGPPSPRRERDRTSSVSISPAKQRRPSEFLNPASEARKMPISNGRPSFSGPGVAHLSTSASMVSPLNPLPSPWTTASDTSVSAFSPSASSTSESALTSGSSNAGGAIKLNRVPTSVRMAADLLRNANTASPVPISGSTPPEHLSPGNEGLVKEQIDQAIMPPPQPPAPSGQYMEDVRPGPTPMTTATPAPSTESHIPLAVGRSPSTPFKTPMVPLLPRPKPSLPRESPALSSNDNVQTPMESAKVHKYRTSLYEPVKSSNLLSESPSESTSGGENGSPAKSDSASVATSPSRFGLGLSIPSGSSTAPFLSPVPQSTTTEKPGIAQLSRLKGAAGEKRGMAGDLAGINGMGDAASHATMIMQSRQAKIQRWGVGSNRGGFAPPPFGRSASAGSPAMLAARRADRQSEWGQLVSGGTAASSPGEDIPATSSLDAFSCAISGTLGPSTSMPSTQTPNPSLLGLSSHGVEDNELRETAGSSTKHQSNAGAAGVGDIEWVDWMDCYKGYKEMKIKAEAEAMRAKTPKASDNPIFETKQLGYEHATRPSLQEERRPAERAHARRFSDQEVTISSQKSGEKIPMTLTLTPTVSRDSQGGSLHGSMKRQSLSFKNLIAVEPRMSPTQRRSSFFERQRQSSGSSTKTDHSYVLGSGSGSGTGLMKKKNLVSKMEGWWNAVKSNFVPEHPPGRPGGERTQGRVNRLPKRSGSIPSITQPESAYLAPEPAALRGSPNLPLRQVISHNELRQRSQRDDHYETASVLDSTPADTARLSRNSSTETAMPIPQHMLVPPPPPVRHASTVTEVTEEPSNPPSRQEPPIHTSLEARRRQPNLRLELEPHTLAQPSPHSSNSSSSQPRMVPLGMPLRLSESSLKSSLYGQSLGPGLTPGAPRWDQTPSPVHTFSQHHVEDKEDKPVAPGAELTVGSVRNHIRRRLNGAKEACDASLRRTVNSITKFVEQERQDVGRQEEVPVDYFETLNLNDSAVFDGEDTGSEAAYENDGPRSRAVSVSWSRGPSRRPSVSLHAISPKARTSALATSPSRIVSRRRPSAVPPRTFGPTGRNVPIPKDRTQSGASSRSTSRSHSPMPGRHISLQVDTTDEDRHFLQALQDLIVLATEVVDSSVHTLKSGLSSCATIIQRIQKIGAKWDEHPDWPGREWYVEILLAVANLGRVLDWWEAEKGFWNFDAETENDQLLFVLKPREEQRFDQEFRAALSEPRYSPLSTAVSVAGDLPSALSLDATSPDVPSPQTTKSEEPGKGLSPKALAIDDLKFLAEHAKSVNIVMELSLQGEIVEYVNDAVLEVLGQVPEEVIDHPITDLLAPGDASVFSEATHQLIEDDNNTVQCRFRFEVHDLAQGENDERQPGPVYIELEGVGMLMRENNEPSHTMWVMKPVAATQVEAITDAVFPRDGHISTEGILCRICEREIVAWFFEKHNETCDALHRLEAEIAECDDCLHELRQTVVDLESEVDTAAPNNPAQYQGVLFFTLPESVAGNDEKAATQYPQGIEVRKIAHEHLQDVLHILSTARQIETPYVREEEADLPSSVQNYLSEESEDRLLKIMRWQRPQTSEFALNLLFTHVEEQLKRKHKAVARMQSTIRYSEKTRHEWEDKVNEMLAEQEDGSGTESELGSGGSEVDKSPSEVHATLPLENPESSPPGPRKIAPLARLPITQAHPHRQPPALEELSDPSSTAPTPAAPQMITTEPAPPALTSASSEPTVPILAPKVRKAPSQVGSKVHSPDVPPPAPSSRVTPSKIGSVKPESRTSSPLLMPYGGDRHHRRRISGSQGMRDAPLSPRIPSAALRSSVAQTTIDDFKILKPISRGAFGSVYLAKKVATGDYFAIKALKKSDMISKNQITNVKAERTILMNQASSPYVAKLFFSFQSKEYLYLVMEYLNGGDCASLVKTLGGLSEDWARNYTAEVVLGLEYLHKRNIVHRDIKPDNLLIDARGHLKLTDFGLSRIGLLNRQVGGPRPAYLRGISLRGSGQHRLSMTRTMSNSSSIDSNMLSPEITSGQSLPNVSQSYFSQMRPSGPEDESSGSESAGIIPKHVRQMATKLSSELGTPSVNGKEPPKFVGTPDYLAPESILGIGQDDAAVDWWALGVVLYEFLYGFPPFHAETPEKVFDNVVSRRIDWHEDEVGISLEARDLINRLLCSDPQKRLGANGAEEVKNHPFFAPINWDTIATSEASFVPDVADPESTDYFDSRGATVGFHDGDGTLQVSKQAATDPSAKINPGAAAMSPNTAGEMEAITNDMAEQSDFGAFNYKNLPILKQANDDVIRKLRTDSMAPRSQALEGAPSLSKGKQRRLSVQVQSKSTRRVSDPNQAPYTPSPSTSTSSAVSTPSRSNMQPPSASTPHHPRRLSELGALDRVRSSDEADGPRKAQSQARLRGDSGSSVGSNSTELTKQRRHASLQLDFAASEPSGGLAENKHFVGSNVHSRGLDVLVAEDNPISQKILETLLTRVGCRCICVDDGPSALAAIMGSIRFDVIICDIHMPVVNGEQVARMIRSTNNHNQLTPIIAATSYEQYINEEGTLFSAVLAKPVTKADLIRCLAKLGFVISAGANDVASMPTPAPAVQS | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 24920 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 7111 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 8364 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4147 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3718 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 17909 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03024 5' flanking region primer 1 | CTGAGTGCGATGATTGTTTG |
| L2 | CNAG_03024 5' flanking region primer 2 | GCTCACTGGCCGTCGTTTTACTTTCCTGACTTTGGGTGC |
| R1 | CNAG_03024 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTTGAGGACAGATTCTATGGC |
| R2 | CNAG_03024 3' flanking region primer 2 | CAGAGAATAAGGTCCCCTCC |
| SO | CNAG_03024 diagnostic screening primer, pairing with B79 | TCAAGGGATAGAAGTTCGC |
| PO | CNAG_03024 Southern blot probe primer | GAGATAAACAGAGCCAAACG |
| STM | NAT#191 STM primer | ATATGGATGTTTTTAGCGAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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