Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02947T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationSCYL protein kinase
Gene SymbolSCY1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNC05570 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
TKL-83.30.0000038
Microbial Kinome Result
PredictionScoreE-value
ePK55.94.2e-18
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K17541 KEGG 2e-207 689.7
Amino Acid SequenceMFAAASNLFSKTSYLSAYTLNSPSPSPSTSSTNLPSTSTPAPSSSSSGYQKSFNVGLWKVTPATHKTTGKEVSVWIFEKRVLEGVKAGVGFGGMGGKEWVLEQLKKEASSLSRLRHPDILHMVEPLEESRSELIFITERVTSSLSSLAAAARSSTNYRSGRPPAAVETSTGGRGEGELDLDEVEIQKGTLQLARGLGFLHNQAKMVHLNLGLEAVVINIKGDWKLTGLSHTTPLTQSDGSATKYVYPEVDARLPPQVQWKLDYLAPEYALDSTLSASNDLYSLGCILYAVHMGGKPPFTNRGSMQTLRDNAEGKLLRQEWASGMGWERCSSELKDLLPRLLTRHPSTRISLLSLPSHPFFSSLAISTLNFLDPTTFASKPREEKATFLRGLVRVVPTFSERLRKGKVLPSLLEEMKDPYLLPFILPNVFEISKGLSKDEFSKVLNKLRPLFTLKDPPQNMLTLLEHLGLFEEKTTPQVFRENVMPLIYNSLESEHFPVQERVLKTIPHLCEILDYGTVQNVLLVKVAIVFTRTRILSVKVQTLDCFRAMVGTLDKATLTSKLVPLLAKIKTKEPAVMMATLAVHEAMGAKVDREATATLVLPQLWSMSMGPLLNAEQFEKFMAIIKTLGARVEAEHTQHLRDVRRIEAQTASLAAANTAALNDGSFGGLGQSNGNGGGDVDFEALVRGKASGMGSYGSVVPTAGVTGVTDPWDDEGWLNDTPSTAMANNDGGGLASAFPGLSVSVNNTGNSFPITTPASVSPRPLSVNTTGTSLKPSTSTTTPAASAKLKARPISASSFNSAAFGSSTSSGSNVLTPTSAGLPPLRPTTSSFSPLQPQIQPQPSRAPSYTSNVSLLGSGPNYNISLTPQSPAQSQSQHQSSPLSFMSPQIPQPSSFSQPALAPTLAPQQQHQQPQLQTPAALAPPLAPQSVVSPPPGWSRGVMQPTAAPKPVWEKSSGGTGALDWGDLDPLK
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 3279
B YPD 30°C, concentration: 5x10^7 cells/ml 2526
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 2436
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 2089
E YPD 37°C, concentration: 5x10^7 cells/ml 2434
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 3480
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02947 5' flanking region primer 1CGTCACCAACAAGTCACAG
L2CNAG_02947 5' flanking region primer 2TCACTGGCCGTCGTTTTACGAGAAGAGGTTTGAGGCTG
R1CNAG_02947 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAACCTGTCTGGGAGAAGAGC
R2CNAG_02947 3' flanking region primer 2TTCCAAGACTTCCCCAAC
SOCNAG_02947 diagnostic screening primer, pairing with B79CCATTACCTTTATGTCCCCAC
POCNAG_02947 Southern blot probe primerTTGCCCATTCCTGTCTTAG
STMNAT#150 STM primerACATACACCCCCATCCCCCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_02947T0
Mating Data
Growth phenotype for CNAG_02947T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_02947T0
Urease
Urease phenotype for CNAG_02947T0
Melanin
Melanin phenotype for CNAG_02947T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_02947T0
Oxidative Stress
Oxidative stress phenotype for CNAG_02947T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_02947T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_02947T0
ER Stress
ER stress phenotype for CNAG_02947T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_02947T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_02947T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_02947T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_02947T0