Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02915T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationAGC/PDK1 protein kinase
Gene SymbolPKH202
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNC05250 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
AGC184.51.1e-54
Microbial Kinome Result
PredictionScoreE-value
ePK266.21.5e-79
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K06276 KEGG 6.3e-152 504.5
Amino Acid SequenceMASSHFGPASPASSTPPPSSAYARLIAPTITRNSSSSSSRSTTTCSSTSSVQAVPMRPPPIETSTAATSRSQLPSNRHSENEAEHDTSYTSPGLSVGGRGGLARNGPRSNRLGTSPQARHVPPSIVALSPSPSPILNMASKRQSNTETVSGTSPSTPLGKSFLAQQDLSPNSSTIPLRVTISVDPNDRLSHDRESHSAERPRSSGNSPVRGRHGHLSTPSSPTNSYRALGGKPRTLSVDAGQNWGGSHRSRARDGDDRERRQSQVSSASSGALKKHSLDDWVLGEELGVGSYSTVYCVTPSANTHSPTSPQPARKYALKVINQAHLIQEKKVKYAMVERDALIRLSDPRPSKGHKRGVSSSSSSGYAQTGSAGKRRSTASIGGQSSMASVSGGTVSNSKKDTRDRLSIVTTSSAASSPVLTASSGSTQLSPTAVSGGGGNIKGRRPSRSAEPPTPVQEQTEMLIRGGEDGKDGQDGQETPSREWDRDRDWDNMTRSRPPSPVREESAEGGEKEKDEEERSGAEPVELGAAIHLTLPPPQIPSTPEPRGSPLLSTDGHRTSRETPRDRPHLTPKRRRQSLAPSERSVKSASTTGKMSAAAHPGVVRLYSTFNDSSSLYFVLSLASNGELASIIRKHGSLDITSARYYAAQLIDTLEFVHSRGVIHRDLKPENILLDEDMRIKITDFGSAKIIAKDEPIVDDSSRSRSFVGSADFVSPEVLRNEVATTASDIWAFGCVLYQFICGKPPFRGATDYLTFQKILKREVEFPKGIDEDAKSLIDTILDLEPNLRPSITFIKTHPFFQSIDFSTLWTIPAPPISSGLREPSKSTTLAQLEASDIWGVFEGSDVGEEDEDGFEYDADTVSPRPEGGAVGEGMMEPLFDRRAAASAVHNVDHPKFSRLRNQYINLGEDLDPPRPAYAGAGTGGRGKREKEVEKKKGEKARGLSHGSESSGGNRSALAGWLEAIKFGGAGGGGMSGSATSVAASDTVRTPGTGTGTGPGSRPGSRAGIPSFGLGPGSGSRSNRGSGASMRSDEARDLSMSLGGLRMNMSKASEFDKWTPLLLANESIIFTSPITLRTSSPALQLHLPAFLLPAPKKRQLVLTDFPRLLMIKDDNEADGDPAGSDSGAGLSSSSHVESGGGGVGGGGRGGGHGSLRIKGEAVFVPRPSTATGSSTTKGGGYSAVPNAVMDVQEKGSKGFTVQTPGMVYYCHVDSVELRAKWMAAIHRVGL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 4647
B YPD 30°C, concentration: 5x10^7 cells/ml 3596
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 3895
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 3971
E YPD 37°C, concentration: 5x10^7 cells/ml 3935
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 5439
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02915 5' flanking region primer 1TGGTGGAAATGGACTGTG
L2CNAG_02915 5' flanking region primer 2TCACTGGCCGTCGTTTTACCAGCCTCGGGTTTTTTTG
R1CNAG_02915 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAGCACGAAAAGCACGAAG
R2CNAG_02915 3' flanking region primer 2TCCTTGGACAACTGGTAGC
SOCNAG_02915 diagnostic screening primer, pairing with B79AGGTGGGATTGCTCAAAC
POCNAG_02915 Southern blot probe primerTGAAGGCGTGCTCAAATG
STMNAT#218 STM primerCTCCACATCCATCGCTCCAA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_02915T0
Mating Data
Growth phenotype for CNAG_02915T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_02915T0
Urease
Urease phenotype for CNAG_02915T0
Melanin
Melanin phenotype for CNAG_02915T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_02915T0
Oxidative Stress
Oxidative stress phenotype for CNAG_02915T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_02915T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_02915T0
ER Stress
ER stress phenotype for CNAG_02915T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_02915T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_02915T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_02915T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_02915T0