Detailed Locus Information for CNAG_02686T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | cystathionine beta-lyase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | GHMP Kinase, C-terminal domain | |||||||||
| Kinase Family | Mevalonate kinase | |||||||||
| KEGG Linkout | KEGG::CNK01740 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MTTPSTPGESSLATSVYSLAPKSPVEVHKERVANWRFSTICANVDGKDQYGASSTPIYQTATFKGMDGQYDYTRSGNPTRGALENHLARLYGATQTFALSTGMTCLDTILRLVRPGETVLAGDDLYGGTNRLLTYLGTHGGVDVRHVDTTDVDKVIPHLGPGNNVKMVLLESPTNPLLKIADLQAIADAVHSAAPNALIVVDNTMMSPYLQRPLEIGADIVYDSATKYLSGHHDLMAGIIAASRPDICKDIAFIINSVGSGLAPFDSFLLLRGVKTMSLRMDRQMATAQLVALYLDSFGFLVHYPGLKSHPKRDIHYKQASGAGAVLSFVTGDKALSERIVGGTRLWGISVSFGAVNSLISMPCLMSHASISAAVRAERGLPENLIRLCVGIEDPRDLVDDLEHSLLQAGAIVPNLQYTPLSQAEAAELYAKDGEAWILERAKGFKRPSTESITVDKLVSSVKQSLGFSTPSKEYKTIDEDIAVSAPGKVILFGEHAVVHGVTAVASSVDLRCFSVLSPRRDGKIGLEVPNIGVELEWEISKLPWNLLPVHANGDRHVADQELDTALLQAVEGAVNTHVEVGKTGIGACVAFLYLYMMISGEETNALSVTFTASSNLPISAGLGSSAAYSTCVAASFLLARQHLTIPSADRLPKEDTDLVDGWAFLAEKVLHGNPSGIDNAVSVRGGAVAFTRSVGGKQGGMDGLFGFSSVRLLLTNTLIPRDTKTLVAGVSAKRLAEPQAVNSILDSIQAISDEARSLLGGGKPVERFILVKRLEALIKENHEYLVKLGVSHPSLEMIVAATAQAPFELATKLTGAGGGGCALTLIPDAFPESSLNELIQTLEGHGFQPHLTTLGGPGLGVLTIPSKLGQASDQLRDAVRTHDEGEGMVVPKRAGLREADKEGLHAWAERLGRWVYA | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 15169 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 10342 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 13042 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 8594 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 9204 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 11065 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_02686 5' flanking region primer 1 | CACACTTTGCTCTTGTCTGAG |
| L2 | CNAG_02686 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACATGGAGATGCGATAAGCG |
| R1 | CNAG_02686 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTGAATCCTCCCTCAACGAG |
| R2 | CNAG_02686 3' flanking region primer 2 | AAAGACGACGCCTACTCTGC |
| SO | CNAG_02686 diagnostic screening primer, pairing with B79 | TGTTCCTCTTCCCTGACAG |
| PO | CNAG_02686 Southern blot probe primer | CACAATCAAAGCGTTAGGG |
| STM | NAT#191 STM primer | ATATGGATGTTTTTAGCGAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |