Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_02233T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationserine/threonine-protein kinase ATR
Gene SymbolMEC1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyPhoshoinositide 3-kinase (PI3K), catalytic domain
KEGG Linkout KEGG::CNE03070 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PIKK314.48.5e-94
Microbial Kinome Result
PredictionScoreE-value
PI3K_Sugen163.61.2e-48
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K06640 KEGG 0 1861.5
Pfam Annotation
Pfam AccessionE-valueScore
PF00454 Pfam 2.5e-59 199.1
PF02259 Pfam 4.4e-23 79.6
PF08064 Pfam 1.2e-32 104.8
Amino Acid SequenceMTQLATQYNIQGADLQSLLPRLLSEGLTPSGDQQQSSPGDLVQVLLEHCILKPLSMSITVNVQQATYTLAIIKRQATLSPRFLTETYNQAPFYQWLIPRLLQIACSSIASVPTDESVQVVVYIIQILGRDQPDDEDTWAKGPGRAAQILSQLTLFCQDVIDGHESKLYGFAELPSNICAVLSIVSAILQLDLPFALSFISTACAQLSEASRLIDTPESQLIYIQTVNKALGRNIHQGLTKACSYVSSIDIVDGADRKIALMTFYEKMASVHEGLKYDVWWSLLRQCGNLDIKDDSIGFPGICHLLTPVGPRLSRDTIKQIVGLDQIKEWEKTAKEISIQTGNSVKLDTVSALLQKDVQKSKKRKRRTSGNLIADLRELLPDLPDPPEGSSIVDILIQNSLSLSAIAHLMPQIVRFAVGEHDWPRYPTSKEFNPKVLELWIKVNVFDLSMMNALSSIIEHAPSGAIAAESQNLEIIYEKVVMGLNARERPMRVAAGRTLSLFFMTQNAGVDHIIARKNQQRYIEAASTPLQSPATAETAVLLLGDLGRLAQGESLCFVLQLLLRQLGSYNSPLRSLAYTELVALAKHHHKTPYTLLSPFLDRISVLLADNIIRSPYMISETMQFIGSTRQNFLHTTLPHILPALVLSRNREALIHVASIVKQRLGRLFMDHIAGILAQVFLHPQQTAESLDFLVDLIRSMTHGYSEHEPLITVESLMGSCMVDLMVILVVELGDQDKAARRAAKLGLSKAMAYQRTGTDLGAFLKPYMLGVISQLNDMLHDVLGKKSVEYKKKIIRSMGVLIKLVGDSMSSFSPQIMASLQSTLGIKELRHETLNTWAVFTSTLKYADIGPFVGRTTGALVANWPTFDKTAKSVAIRIIDEIADSANDLSQFVEEVVGMDHIDELQRAASLLTAQRKKWPIDIRITKVLDRVASKNIAISLASIRELRLLLVTLQESIQDLVKGDTFNPVAARLMSTLLSIATRDGDCQELRDLSYECLGIIGALDPDRLGYYVESNTFTITSNFADHKESLDFALHLVRDLLVDAFRATNDTKHQNHLAFAIQELLRFCGFSLKVIHPASKIDPSIRQRWQSLPKDQLETLTPLLESRFTLHDVSFRTFSHPIYVAAPTYREWLQRWATDLISKVMSMPDTDRSVSDSKAIFGVFCGVLRNQDVSVAHHILPHLVLNVLLSGVREYRDEICLEIKTVLQDQVQPTSPADRRSLSAQVIFDLMDHLSKWLRLQRVKGSHLDRGERSKVVEGVLSSIETELMAHAALQSKAYARSLRSFEERIIQLREEKKDTAELQTYFERLHQIYAELDEPDGMEGVSAFVISPSLEHQIREHESTGRWTSAQSCWEVRLQQSPDDPTLHVGLLKCLRNLGHYDTLRTHIRGVITRHPDWSLQLAPFAAEAAWIIGDWDTVRQVGPDCPPIGQALLALHEDGDLSSVLTRVRREVGAGITGKGYTPVYEALLQLHLVQEIAMIQDTKKEIQIVSKSKNRHKVVQQHVRQLTASLDSRFYTTSPAFRVREAILSIRRTALGLMNTPSLNPEIGDAWILSSKIARKAGYEQTAYSATLQAREADAPFAFVQEAKLRRAQGSVFKALTDLQNTLAPLATDSKASENIEQDSFRRSRDLAKAVLLLARWANETDRFDQNEIVKRYTQAITLCDTLESPYYHLGHFYDGQVGDPAQKIIYNYHTCNYYSIALRHGVKYIFQTMPRMLTLWLDLGDTKDAKKKKFISKIHSVVGEAAHDLPAYQFYTAFPQIVSRIVHPSVDVSRILRSIMVRVISEYPQQALWPMVGVMKSCQDERRNACLAVFQKATSISTIIRDADTFSSTLLKFTDHKVDGRKREKSIQSHFPYVKSAFPTKMILPLQDALTCNLPTSSDTVKTHNPFPNAPIEIHDVEDRVDVMPSLQRPKKLVFIGSDGKAYPFLCKPHDDLRKDARVMDLNSMINKLLKSASESRRRQLYVRTYAVMPLNEECGLLEWVTNTHGFKGILETNYGRQNKKIFINEVIDLLTTTRKQCPPEVLTAVFKDKVLPLYKPTVFREWFLTSWPEPSAWLSSRLAYSRTLAVMSMIGYILGLGDRHGENILFDGLSGDTVHVDLNCLFEKGKTLEIPERVPFRLTQNMVDALGVTGVEGVFRKAAEITMSILRSNSDSLMSVLEAFVHDPLVEWTSRGRGKSDPRDIRSNADKNLHPIKRKLRGVMNEGTVVSVPNQVETLIKEATSPRNLGAMYVGWAPWL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 12525
B YPD 30°C, concentration: 5x10^7 cells/ml 7996
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 8571
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 7159
E YPD 37°C, concentration: 5x10^7 cells/ml 7003
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 8835
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_02233 5' flanking region primer 1TTCCTCATCCACGATACTTC
L2CNAG_02233 5' flanking region primer 2TCACTGGCCGTCGTTTTACGACAGAGGTTTGAGGATGC
R1CNAG_02233 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTTTTGTCCACGACCCTCTC
R2CNAG_02233 3' flanking region primer 2TCATTGCCACCTCCACCAAG
SOCNAG_02233 diagnostic screening primer, pairing with B79CTGATTGAAGGAACTTACCTCG
POCNAG_02233 Southern blot probe primerGGAGAAGTTCACGAAGGTCTG
STMNAT#204 STM primerGATCTCTCGCGCTTGGGGGA
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_02233T0
Mating Data
Growth phenotype for CNAG_02233T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_02233T0
Urease
Urease phenotype for CNAG_02233T0
Melanin
Melanin phenotype for CNAG_02233T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_02233T0
Oxidative Stress
Oxidative stress phenotype for CNAG_02233T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_02233T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_02233T0
ER Stress
ER stress phenotype for CNAG_02233T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_02233T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_02233T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_02233T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_02233T0