Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01988T1
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationbacteriophytochrome histidine kinase
Gene SymbolTCO3
Kinase SuperfamilyHomodimeric domain of signal transducing histidine kinase
Kinase FamilyHomodimeric domain of signal transducing histidine kinase
KEGG Linkout KEGG::CNK03200 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K11354 KEGG 4.7e-139 419.1
Pfam Annotation
Pfam AccessionE-valueScore
PF00360 Pfam 9.4e-28 94.4
Amino Acid SequenceMPDGSTSPREPANSAHATHKPQSNPSPPQSVAMPAVSRHVQNASNQDHGSSSTPVSPAASSAGPLSPNIQPGSFVFPIRSMFQGMVHSDSSNSMTEGHQHQIQDSLQRSRSGSSSPLRSPAFSDVRRFSTNAAGALHDEPDAGIQAIAQMLQQDKDNALKEKGKNRPGVATFVGKAERDHRGSGGEETLKPSPDASGHLGNSPVTSSNGDSEHLKQHDVVKSSHFEEGANDSPDSSLGSGGTAKQATPTDLPTTLQRSSTLKGKASTSSHTETSPFNYCNFPSEHHSGQRERRFSTLNKVNPPSCNSSSHPTDTSHPPNPELQNPVPQHSTRQQHTQAAEMNNRTSSHGSRTSLEVDESGMRSLISDMSGIVLLGEAGSLSSMGGSGVSGSKGTNGGAGMTGTVDSASIDSAHVTGGGGQGDDRQTARASPATQHRRARQTSRQDSVRSFIQSQASTTNLQDPNAPTPSPMGNGSPSAEDSQDAAVSMEDSASNEKQREQQQEEEEAGQHGEVENEQVEQPVAASAEDAENPDEPAVTMRFEHVTTEEGHHLVAGREGKLSRCQDEPITTPGAVQGFGVLIVLEEDYETGNLEVRQVSENSTEILGLSPKYLFKLDCFTRLLSSDQETILRDILEYLPESYGESNSVEDSGPSIFLLSGFGEPGSDVEESKTMSEPEAEVRSIASSAGGKRKEWTCWVAAHRPEHRGWNKVDEKGEPIPPPDWIILEFELERDVYNPLVHPSEDAETSAAAASSTARSRSPDSTAASVSASGSNSNSNTLSASTRSGERTLDSLTSTLAGDRAGVSVALGSGTASADASPGDFGSSASDLTSVPKQEERMGLDGLEMHIPLEKILESTTNHASPLRALERMRGTTAKASNEPRSRGRGRGGRQRPVRTKSGGTGTMDIFAILGQINDQLGAAPDLDTFLKVAVGLMQDICRFHRVLIYQFDEQMNGLVVSELVEWGKTTDLYMGLRFPATDIPPQARELYKINKVRMLYDRSQTTARMVLRDKEDLDQPLDMTHCYLRAMSPIHLKYLGNMSVRSSMSVSIMAFGQLWGLIACHSYGRHGMRVSFPVRQMLRILSDSISRNIERLSYAQRLHTRKLISTVPDRSHPTGYIISNAGDLLQIFGADAGLLVIGDGCKLLGNNEQGQAMLAIAEYLRIMRFDNLKASSSIQRDFPDLILPRASDTIAGLLYVPLTAKAGQDFIVFLRKGQVREVQWAGKPYKDGKASEEASLEPRKSFKAWTETVTGCSRAWTDDQLESAGVLALIYGKFIQVWREKQTAMASNQLTAILLSNTSHAVRTPLSQIINTLELALAGNIDGDVRKMLENSHQASRALLFHVHDLLDLTRIETGNETAFNDPFDIRQSISDAVRLYQTEVARRGLEFRVNMAEDLPQYVVGDSRKIRTVISNLVANSVKFTEKGFIEVYCGIQRPPDEESSKEASLVESKEGNTVTIEIVVSDSGCGIPTVQLEEMFVTLEGAEPLQKNTGVGLGLAVVARIVEQLEGQLRAESEVGVGTRFYFNLPMVSHPSGRPGSRQSSLNSRNTNSSQLRTRTGSSGSKSDSVVSLRSDGSGVPEIESFVQDFGGSHLPAPVGDDDPRLLEAQERMNRLGTFPVTDSSYPIRATKVNSEDQHVKSPKPKKPRFTAVGSSKRPTSLHNTISSKTSSSSQSPAFSRPSSSSVQVPKRNKKGPNGKTVMRIMVVEDDPINSQILQKRLKMDKHAVVAVTNGQEAVDQLEKDRDIDAILMDIQMPIMDGRTSAKEIRELEARTPQPDDIEPFKVDGRTPIFAVSASLYEDDRANLAENFDGWLLKPLDFSRVRAILEGLESSEKRAAEVYQQGNWERGGYLKGESHFQKISSQLH
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 16262
B YPD 30°C, concentration: 5x10^7 cells/ml 11464
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 13185
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 8301
E YPD 37°C, concentration: 5x10^7 cells/ml 6771
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 11756
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_01988 5' flanking region primer 1CCCAGAAAAGAAGGTTGG
L2CNAG_01988 5' flanking region primer 2CTGGCCGTCGTTTTACTTGTGGTTTGTGGGTAGCGTGG
R1CNAG_01988 3' flanking region primer 1GTCATAGCTGTTTCCTGGGCATCATTGCTCATTCTTGTG
R2CNAG_01988 3' flanking region primer 2AAAAGGTGAAATAGGGGCGGCG
SOCNAG_01988 diagnostic screening primer, pairing with B79 TGTTCCTCAATGAAGTGTCC
POCNAG_01988 Southern blot probe primer 1ATGGGGAGGTCTATGCGTTAGC
PO2CNAG_01988 Southern blot probe primer 2 TAAAGTCTCTTCCCCACCCG
STMNAT#119 STM primerCTCCCCACATAAAGAGAGCTAAAC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_01988T1
Mating Data
Growth phenotype for CNAG_01988T1
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_01988T1
Urease
Urease phenotype for CNAG_01988T1
Melanin
Melanin phenotype for CNAG_01988T1
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_01988T1
Oxidative Stress
Oxidative stress phenotype for CNAG_01988T1
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_01988T1
Cell Wall Stress
Cell wall stress phenotype for CNAG_01988T1
ER Stress
ER stress phenotype for CNAG_01988T1
Genotoxic Stress
Genotoxic stress phenotype for CNAG_01988T1
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_01988T1
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_01988T1
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_01988T1