Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_01687T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationAtypical/RIO/RIO2 protein kinase
Gene SymbolUnknown
Kinase SuperfamilyWinged helix DNA-binding domain
Kinase FamilyRio2 serine protein kinase N-terminal domain
KEGG Linkout KEGG::CNC01960 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
RIO-71.90.00000011
Microbial Kinome Result
PredictionScoreE-value
Rio375.71.6e-112
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K07179 KEGG 1.1e-190 634.1
Pfam Annotation
Pfam AccessionE-valueScore
PF01163 Pfam 3e-52 174.6
PF09202 Pfam 1.4e-32 109.4
Amino Acid SequenceMKLDATDLRYISADEFRVLTAVEMGSKNHEVVPSQLVAELAQIRGGNVKKALGDLAKRNLVARVQNVKYDGFRLTYGGLDYLALRTFSRRKPPSVHSVGTRIGVGKESDIYVVADERGEKRVLKMHRLGRISFRAIKSKRDYLGKRKSASWMYMSRLSAQKEFAFMKALYQHGFPVPVPIDQARHCVVMSLVDGYPLRSVEAVDDPADLYSKLMELIVRLAHAGLIHGDFNEFNVMLLRKTGEPVVIDFPQMVSTRHENAEYFFSRDVNCIRRFFRRRFRYEATSWPTWKNVLEIETGNAENAVQGGVNDGLTDLPVAQNRVRIDLEVEASGFGRALQRELEDYMIEVQDIPGSESDEEEDGEEKEEEEEEEEEEEEEEEEEEEEEEEEEALSSHVQGNDEKELSDEAAVAARLEALRLHRALGNDVSDEDADPDSEHSLTDFTSDDDSDDSDNSTGVAQTDFTTYTPSMRNQPRHHTRLPVKEFENRGGARSAIAKQVERERHQTERKVNAGKTSVKLGKQKGHKWKSSDKYVVGKDSGW
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 2683
B YPD 30°C, concentration: 5x10^7 cells/ml 2193
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 1685
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 4400
E YPD 37°C, concentration: 5x10^7 cells/ml 4850
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 2336
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_01687 5' flanking region primer 1GCTCCTAAATACCTGCCACTC
L2CNAG_01687 5' flanking region primer 2TCACTGGCCGTCGTTTTACCTCATCCGCAGAAATGTATC
R1CNAG_01687 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGTTCGCTTATGGTCTATGG
R2CNAG_01687 3' flanking region primer 2TTGCGACCTTTTTCTCGG
SOCNAG_01687 diagnostic screening primer, pairing with B79TGTTAGAAAAGCCTGTGACG
POCNAG_01687 Southern blot probe primerCCCAAGATAGTCTCGTTTGC
STMNAT#290 STM primerACCGACAGCTCGAACAAGCAAGAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG