Detailed Locus Information for CNAG_00266T0
| Species | Cryptococcus neoformans var. grubii H99 |
|---|---|
| Gene Annotation | hypothetical protein |
| Gene Symbol | YFH7 |
| Kinase Superfamily | P-loop containing nucleoside triphosphate hydrolases |
| Kinase Family | Extended AAA-ATPase domain |
| KEGG Linkout | KEGG::CNM02590 |
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. |
| Kinase Prediction | N/A |
| Functional Annotation | N/A |
| Amino Acid Sequence | MQVTPEQLVNEIVKKYNELPKDRRLLVAIAGPPGSGKSTLAYPLADALNSFILGHPPTNPSHIETPVSSLLAEGSSQQGNGEEVALTIGLDGWHYRREELDGFDDPQDAHWRRGASFTFDLNSYKAFLLLLRLPLCPHPPKNIPFPTFDHASKDPKPSPFPILPGHRIILIEGLYTLLDQPGWRDCAAMMDIGVWVDVDEKVARKRVIKRNWEAGIVEDVKKCEERVDAVDMKNGEQVRNYLASPTYIIRSIDGELLSKESFRFD |
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1506 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 2516 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 2457 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 2037 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1513 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1458 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_00266 5' flanking region primer 1 | GGTCGTATCTCTCTTTCAAGC |
| L2 | CNAG_00266 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTTGACGAGTTGTTCAGGGG |
| R1 | CNAG_00266 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTGATGTGGATGAGAAGGTAGC |
| R2 | CNAG_00266 3' flanking region primer 2 | GTGCCGACGAGAAGATAAC |
| SO | CNAG_00266 diagnostic screening primer, pairing with B79 | AAGGGATAATGGATGACCAC |
| PO | CNAG_00266 Southern blot probe primer | TCAGTGAGATTCAAGGATGC |
| STM | NAT#295 STM primer | ACACCTACATCAAACCCTCCC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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