Detailed Locus Information for CNAG_07744T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
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| Gene Annotation | Atypical/PI4K protein kinase | |||||||||
| Gene Symbol | PIK1 | |||||||||
| Kinase Superfamily | ARM repeat | |||||||||
| Kinase Family | Phoshoinositide 3-kinase (PI3K) helical domain | |||||||||
| KEGG Linkout | KEGG::CNG00610 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MSHALLLRLFLSPYFSISIAMQYLKTYPDSIGISHYLCWRMKRMPAEEVEFYWPQICHLLLTYPTQSNALESFIIARAEESTHSAMLTFWFMQAALRDLIPTRQTDPVPFAICQRVLHRCHAIIFGDPPEPSRSPYRSLPHSPSSSALGKLSSIPGSGSSSTFIQIDEGQRQGQGLGQGRVNPHAAAALVGMGVMFAGIPGMPALVPLVGEWALIQGRRPMDDLTSGRDRVEVDTGGGADVRPEKMERRRKENENERGEKEDSESEEEDGRAPSNGIIQGQGYKSNVKFPKSTVNLAITGPAQTTPNLYSPPRSPSAVPPFSAIDMPDEKVREKEKNGGISSRRKGSDPFGQLFNDRSPTPPKSAHQPFHSVPSLPRSSSYNPNHYQGGPPTAEAILATYSFSAQRQLLTSHYCRSEVRFLLLLEDISNRLLVIPKPARVSALRAELTSLNHNLPAEVCMPLWCTADHTLSPSSSSSTLSPSPASGSNNPSSSAKNRAYHPRGSAVPGGHSRVVRISPGDSVVLNSAERAPYLLHVEILQGDLNFDPTRRENRELLKKIVVAEDRKRRKRDGTLGVGVDGREAGSGGRVRNAGYNNGRQEWGLGSKKGKGKGEGFGSLMPDSIPAIPPSPSPASASISNSSKPPSQPPLTATTTTPGTPKPIPPEEMDLVEQLYGTLSVHDEIPDLSEIIPLPSGPKNRDLDMKAWDRGSPVPGRITSSGVGVDAGAGIGAGVMGDVGQWMSSPTGTATGSEVGKSGPSTPGFPSAKSGFTSPSPSSSSPFTPGLVQAQGQSPSTPGPKRAITLEDYSERMRTAAVMLAQLNASLVPNAPENQSGGGWIPGTGWIRTPGGGGGGGGEEAGGKPDGSAGGKLKLAAAQAAAIRERIMLEMMALEEERVERMTDRPPGADVPIPTAETSGGGESGHEHDHEHGTVEDEGIVRRELNKADPSAAVFRESWSAKKSRIRAGSPWGHLANWDVISVIVKTGADLRQEQLATQLIERFSRIWKEENCDCWARFFRILITGETSGLVETVTDAVSVHSIKKGEYAKRLAEGGPIGHVSLMDHYVNTFGKPDSGRFARAQRNFIRSLAGYSIITYLLQIKDRHNGNILVDQDGHLIHIDFGFMLSNSPGNMGFEAAPFKMLMEYVDIMGGLNSPGYAYFKKLFKEGFEAARKHSDSLITIVELMQKNSKLDCFALFGDQTAAHFRERFQLGLTTQAVDAYLERLIVTSTGSNYTKLYDTFQYYSQGVL | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 4001 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 5393 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 5926 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4171 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 4421 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3964 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07744 5' flanking region primer 1 | TGGTAGTATGCCAAGAGGTG |
| L2 | CNAG_07744 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTGGGATACTCTCTCTCTCTGAG |
| R1 | CNAG_07744 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAAAGGGCAAAGGCAGAAG |
| R2 | CNAG_07744 3' flanking region primer 2 | GGAGATGAAGTCAAGATGCG |
| SO | CNAG_07744 diagnostic screening primer, pairing with B79 | TCATCTTCATTGTCCTCCC |
| PO | CNAG_07744 Southern blot probe primer | TAAAGAGCGGTAAGGCGAG |
| STM | NAT#227 STM primer | TCGTGGTTTAGAGGGAGCGC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
| B7507 | CNAG_07744 PIK1 complementation LP for 5'-exon region | gcggccgcTGGTAGTATGCCAAGAGGTG |
| J339 | CNAG_07744 PIK1 complementation RP for 5'-exon region | GGTACCGTAAAGTTGTTCGA |
| J338 | CNAG_07744 PIK1 complementation LP for 3'-exon region | ggtaccgcggccgcGCTCTTTTCTTAGTCTGGGG |
| J340 | CNAG_07744 PIK1 complementation RP for 3'-exon region | GGTACCCTCTCCGTTCACGA |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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