Detailed Locus Information for CNAG_07427T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | CK1/CK1/CK1-D protein kinase | ||||||||||||||||||
| Gene Symbol | CCK2 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CND04310 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MTSMDLRVGGKYRIGKKIGSGSFGDIYLGVNIVSGEEVAIKLESVKAKHPQLEYESKVYKTLAGGVGIPFVRWYGTECDYNAMVLDLLGPSLEDLFNFCNRKLSLKTVLLLADQLISRVEYIHSRNFIHRDIKPDNFLMGVGKRGNQVNVIDFGLAKKYRDPKTHLHIPYRENKNLTGTARYTSINTHLGVEQSRRDDLESLGYVLMYFLRGQLPWQGLKAATKKQKYDRIMEKKMTTPTEVLCRGFPHEFAIYLNYCRSLRFDDKPDYSYLRKLFRDLFIREGFQYDYVFDWSLQPGVKSSGTGGDDGMQAQDNQRQLRSQTKARDTGGW | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 23124 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 17127 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 15691 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 15849 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 14460 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 18212 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07427 5' flanking region primer 1 | AGATTCACTCGTCATCGCC |
| L2 | CNAG_07427 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTAAGATGCGATAGGTGGGCG |
| R1 | CNAG_07427 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCAGACTAAAGCCAGGGACAC |
| R2 | CNAG_07427 3' flanking region primer 2 | GGAAGGTCAAGCCATTAGC |
| SO | CNAG_07427 diagnostic screening primer, pairing with B79 | TCAAGGCTTTCATCCCGAC |
| PO | CNAG_07427 Southern blot probe primer | CGAGACCAGTTATGTTTGAGAG |
| STM | NAT#230 STM primer | ATGTAGGTAGGGTGATAGGT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |