Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_07408T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationSTE/STE20/PAKA protein kinase
Gene SymbolUnknown
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CND05790 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
STE348.64.4e-104
Microbial Kinome Result
PredictionScoreE-value
ePK311.63.4e-93
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K19833 KEGG 2.3e-261 629.4
K19833 KEGG 2.3e-261 239.6
Pfam Annotation
Pfam AccessionE-valueScore
PF00786 Pfam 1.2e-26 90.4
Amino Acid SequenceMAFSPSLTPSRPAPVPPRRAAITAPYIVRVTDASAAPQSNFSATSYSPSFSYTNSSIPSTKVLDTTRSRTIRSGAASIKEEGLRAFMWSKRWLVLGSAELRIYKNESSPSPVYTVSLEDIQDVQRVDMKPYCVEIETSDKLLYLAFRSDNEVYGWMDDIYSRSPRLGVSQPTNFVHQVHVGFDPKSGGFTGLPPQWSKLLTSSAITKEEAARNPEAVLDVLQFYTQQQAGNVLEGYQQFPMPTVSNQSLTTGAAATRFEGVGLGGQQHQQQQQLRSAPFTAPGSLEAITVVPARPSFETEEKTSAQKEKEKVLNPQHQLLESTRPAMAKFDKEKVTRSPGPTMEKRISTMNEVQIMEKLRSVVSAKDPSQLYSKIRKVGQGASGMVFVAKTLLNGKKVAVKQMDLSQQPRKELIVNEIIVMRESQHPNVVNFLDAFLVRGSELWVVMEYMEGGALTDVIENNKLEENQIAAICLETCRGLQHLHSRSIIHRDIKSDNLLMNTQGEVKITDFGFCAKLTDQKSKRATMVGTPYWMAPEVVKQKEYGAKVDIWSLGIMTIEMIENEPPYLDEEPLKALYLIATNGTPTLKSPEKLSQDLKHFLSVCLCVDVAFRATSTELLKHEFLQLACSVRDLAPLLRFKQDTL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 5884
B YPD 30°C, concentration: 5x10^7 cells/ml 6843
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 7269
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5952
E YPD 37°C, concentration: 5x10^7 cells/ml 6037
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 4952
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_07408 5' flanking region primer 1GCTGGCATAAAACCGTTC
L2CNAG_07408 5' flanking region primer 2TCACTGGCCGTCGTTTTACCTCTTACTCCACATAAATGCCC
R1CNAG_07408 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTTGAAGTCACCCGAGAAAC
R2CNAG_07408 3' flanking region primer 2ACACTGCGGATTACGAAGC
SOCNAG_07408 diagnostic screening primer, pairing with B79TGTGGCTGAGATGAGGTAGG
POCNAG_07408 Southern blot probe primerTCTGGGCTGAAGTCTACTAAAC
STMNAT#6 STM primerATAGCTACCACACGATAGCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG