Detailed Locus Information for CNAG_07377T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | transformation/transcription domain-associated protein | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | |||||||||
| Kinase Family | Phoshoinositide 3-kinase (PI3K), catalytic domain | |||||||||
| KEGG Linkout | KEGG::CNA06110 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MASSAPSTNRSGRPRKSSTATPHPLPLTATPSTETPAPSAAGGGMSMPPPSYTSNGPMSVSDCEFLASQLLEPAVTARKKLEIALELRDSAENNRDFGFYDKYLSIFIPALISILGDEKSITFVKDNVEQRFRHTLLAFLQRLPHTEPFRHHMSSVMELCVKLLKIENEENALLCIKIMIDGLRSNKDQMEPFTEPFLDLVKQMYANIKAVVEKEFGPSGGGPKSVSTQGEGSANGQQSQQQQASSSNHTILPHALHSPKVLTECPIAVVLIFQTYKSIMQTAMLDFYPLVIDSIKIQPEPQRLAHLEAKEKGEIFVGVASGITNREMFAELVKAQVKTMAFLAYVLRGNQGSNRDYVNVFPEACARLLRDCPPEDVITRKELLVATRHILTVDSRSSFIPYIDVLLEERVLVGTGVSSREMLRPLAYSVVADLIHHVRNELPLQQLIRVVYVFSCNLNDSTFSSSIQTMCAKLLNTIIDSIYNKADTNEMSKILKGMFFTFLEKLSAMSDAHDRLKALAARDKGKGRAKEEGDEDVEVTDASDEASDKLIHGWRDIEQAMPVHSVAYANESVDSFCRDSRYLFKTILHTFRTLLSYTRQGENPPPQPDGEVLSRFFECSIKCFAIFDVLNRDPREAKEALELLSEIVLLFEPHVFAEVWTSHMEFFSDISITNNQVFSLLQMVITHESVSHQLVSILLKYLMEKLPEVGRMDKQRATLMLKMFKIAFLAINTYITSNEAVLVPHLQKLIMSSFESAAKAEDSSFYYQILRALFRSIGGGRFEALYKEVLPILQEMLDHLAYLLDHSPDEISKDIFVELMLTVPVRLTNLLPHLSYLMKPLVRALSAGPDLVSQGLRTLELCIDNLTADFLDPTLAPVLRDLMAALHQLLKPIPANREHASAALKILGKLGGRNRRFQEVHDNLEYRLLADRLVVPITFEGTRHLLDLTPLVNSAGKAIDNEADLLREDGLQVLMYSALTIFEKGAPGPEGNATFRTNMTRLFFACDRPIIGERALIFVRDLCRRAFALELGRTDGIEHPIKPGPDHSRRRFLPLTNALSDAFLETLTISKAAEQKGLSDLLATIVMDFKELALSPRFQGVVDGHRSFDRMVTFFALRLVTLCHEEAWSKKMAGVSAISSFAHKIELSRKNIIDLQLDFVRALLYCLRDAPKDVPRSADDVIGLIKHLIRTCQSQDDGKPRIGRLIETFVGELNSQSKLARDAAQQCIEVLAEVTAQTVPELITNIAKVKLLSVDHGPIYSKPLRALPFAMQVGNISAVTYLMDLRPSVVETSEEFIRLLHEVLALADVDDANLVSKPATHKQESWLKALRICCLRLLKSSMATPDFMNKPTQGQLRARIIQVYFKHVYSQNPEIVAVAHEGLRDVLQQENRLSRDVLQKGLRPILVNLADAKRLSVSGLDGLARFLELLTNYFKVEIGVKLLDHFKTLGDHQMLVKAAYAPLDDNHNIARMSRLVNIFRLLPSSAIQYLNDLVANVVEVEALLHQSQPGPFTEYLGRYLDRYHANAVQNLFDNIRNTRYVWTYRNIITSGSAPHLVEEFASRGEALCQLCFSNPEVTDLVLPGLLLVRDLSRVQSSWLSDSEPVLEPMVNVWRMIVNKSRDPKADITGYEFQQIPSLLLEMFMASLEQQQHIPLLFHVVEAYEVRAAFERSHVTFFLYRQVALQESVEYRREVIEYFFSLYEAEDVPWTYKTNALRVIVNPTLRVYFGDPNHDGSLISAQLVRKIANLMWRPLSATTSSKQREDTHLIEVFALTTMLVQHCGAKVNEARKEIFKLAWMGINLLEPTVKLMAYVLAARFMATYDTPVKFVRLTWTGVLRLKDTDNRVLYRQAIDTLASSLSVRDPPPANGTPEWAKLLRTVLIEEGHATNQLVTVCELLVHHPDLFYDYRELYVPHIANSLGKLAFAQAATPELKKLTVDIVELIFNWEKRRMAARDETMDMDEGPKRGADQSAEQGPTKKQRVDRAGTAVSGSSGGGWAAPSQVRELMTAHLLRLVSTSADPVTRNGLTKRALMLFKDILGPKGLPNVHVKLGFFHRTMTQDINPNTRPTVANSTEVIAAVAAAVKDTQWVKANLSLLSKLLEKVWVSPETDLHEVVAPLTEDLFSEMPADEGAEAEPDAKALLVFVQTAVNDGLSASLRSTLSLPGTLFLLKIWLKTHPRVLQSEGISSALLKVLANLIKLHTTSNQPANTANEPDIVRLITSVLDILRDRVNDLREQRKHLYSSITILVDRSLNPVLCRYLLQLMRHWVIGSSDGAAHGKEKAMILLRMMCFESRNDLLFQEFLEVVYDVYQQENLRGSDITHRLEPAFLLGTRSKNAEQRARFLDKLEQNLPRSIDSRLQYLCSLQNWDTLADSYWIPQILSQLLGVADLGQSLTQQPMPRILDLDPIVDMAESACIRHIIRPARNLIHIDVTLSHDLWVSVFSMCWGSLSRSHQLAFTPYLIKLLSKSHLQRQTEMRPNVVQAFLDGIAACTVPITLPPTLVRFLAKNFNAWYVGFEILTRLTDVYRGDDGLRETCASALSELYAELCEEDMYYGVARSRCQFPETTGALTYEQNGLWPKAIELYEQAQIKARNNMLPFSEGEYCLWEDHWILSAQKLQNWENLTELARIDSDADLLLECAWRLSDWASPDREAIDQNLARVIDHPTPRRKTFEAFVALLRSHMAREPPNEFLRVLDEAQQVTLRKWISLPPHMTNAHLPLLQMCQQIVELGEAARVFDSLQMTNQANLELRCNSDLKPIFQTWRDRLPNFWDDISVWSDLLAWRQHVFQAVTKVYHPLVAQPDNATYGYRGFHETAWMINRFGEVARRHGLLDVCSVSLNKIYMLPNIEISEAFLKLREQALCFFQKPEKFNEGLENISTTNLKFFGLSQRAEFLTFKGMFISRLGQNEEANAEFAHAIQTDWNLPKAWAEWGRFNDNLYKDRPENPATGPPEPEPGKPKMTDAQWQESYSQDRAILASSAVSCYLQAAGLYNNHKSRGLLLRVLWLLGLDDSHNTISKAFENYKGDLVIWYWITLIPQLLMSLSHREASHARLVLMRIAKSFPQALFFPLRVSREDFVNVKKQQQMQQRFAAARRAENQAKIAAANAIADASGQPSETKDVKDEQSAANATGIQPSASNGPAMGLAAQTQSPSSQTPRQPWDHVEEIMNMLKTAFPLLALTMEKMVDQISLRAKPASDEDIYRFFSALLADAMQQWGGRSGLPNDDGELNAQTKDNLAKFATNLNGELKVMIEKDFMVEMPKLREYIRRLQRWRDLYEKNLDDRSKTLPLDQGGCNLTEFHHTKFDDVEIPGQYVQHVDQGEEFIKIARFAPRAELGRGHGYCFRRITMIGNNGVTYTFHVQMPAARHCRREERLTQLFRIMNSVLWKRKESRRRSLQIHLPTATPLAPQLRLVQSDSSYVSMQEIFEDFAASKKMAREDTVLTYFDRIKELHDPAIPRNDHRYIQLRAELMEEIRVKMVPETIITNYMIKSMNGPENLWLMRKQFAAQTATTMFLTFVCCLSNRTPSRFYISRKTGLMYMSEILPAFAPGQPLINSSEAVPFRLTPNMQHFATRAGVEGVITGTCTAMARCLTAPEFDLSGTLSLFIRDELLIWHNTYMKDSRLESPLLGHVYKNVDSFIRRVSTMGFIGENRDKSSNAPPVVHAIISLISQATSALNLAQMGETYMPWY | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 32802 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 15100 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 16197 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 12039 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 12597 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 17495 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07377 5' flanking region primer 1 | CGATAACGCAACTTACGG |
| L2 | CNAG_07377 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTTTGGCTTGATTCTCCGC |
| R1 | CNAG_07377 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCTCTCAATCTCGCTCAAATG |
| R2 | CNAG_07377 3' flanking region primer 2 | CTGAGCCGATAGAGTTCAAC |
| SO | CNAG_07377 diagnostic screening primer, pairing with B79 | ACCAACGCACATCTACCTC |
| PO | CNAG_07377 Southern blot probe primer | TTATCTACCGAAGTTGGCTG |
| STM | NAT#296 STM primer | CGCCCGCCCTCACTATCCAC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |