Detailed Locus Information for CNAG_07372T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase | |||||||||
| Gene Symbol | OXK1 | |||||||||
| Kinase Superfamily | P-loop containing nucleoside triphosphate hydrolases | |||||||||
| Kinase Family | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, kinase domain | |||||||||
| KEGG Linkout | KEGG::CNA05980 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MAAPLYVTQSGRLWHAGLILIVTVGLPARGKTHISRALERYLRWLGVKTRVYSIGDYRRKVLGGAKDVPHDYFQTKTPRSEATNALRRRIKAELEDQIMDFFTVQGGQVVIYDANNGSVAERKSTLEKFGNKGVHVIYLESLCDQEDIITANIRSVKLSSPDYAGWDAEKAVADYWERIRDQTEVYDTVTADEGPFIKVMNVGERIEVNRIEGYLQTRCCFFLMNIHTRPRNIYFARSGQSLIEHSYKADSDLSPAGWEYAERLKAAVIARRKAAREERKAKGEVLGEENPLLIWTSARRRAYHTAWPFVHSGYKVVQKPIMSEINPGVWDGLSTQEAMELYPDEWTRFLADPYAHRAPRAESYHDLSVRLESVIFELERCQDDLLIIGHASVIRCLLAYLVGLPPNEVPAVEIARGDLVEITPASYGVISRAWHFWSGEGRGDAYGENLYENFAEATSGKGSVLPDSGVNFAADALNMEKEAEEEEGREKEERGEKVMEAAKAVGKIAEQAAANTAAGAGVANSSGDGAGAGGVGQGARGPGKALKRWGSERGKGRGLPGLSELNETDEGEENKDKDDGGVVSEGEGEGRESGKTRSRAMSLLEI | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 4565 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 3664 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3618 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3373 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1991 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 4871 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_07372 5' flanking region primer 1 | TGTAGTTGGGTGTGGTGTAAG |
| L2 | CNAG_07372 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGGGAGTGAACGAAGTGAAG |
| R1 | CNAG_07372 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGGCAAGACGAGAAGTAGAGC |
| R2 | CNAG_07372 3' flanking region primer 2 | GAACCTGAACCTGAACCAG |
| SO | CNAG_07372 diagnostic screening primer, pairing with B79 | CTTCACCTTTACCTTTACCTGC |
| PO | CNAG_07372 Southern blot probe primer | GTGTGGCTAACAGTAGTGG |
| STM | NAT#122 STM primer | ACAGCTCCAAACCTCGCTAAACAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|