Detailed Locus Information for CNAG_06432T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | acetate kinase | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| Kinase Superfamily | Actin-like ATPase domain | ||||||||||||||||||
| Kinase Family | Acetokinase-like | ||||||||||||||||||
| KEGG Linkout | KEGG::CNN01560 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction | N/A | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MPDKAEYLLAINCGSSSIKGKLFAIPSFELLANLAVTNISSSDERVKIKTTWEEGKGKDSEEEADYGDKIRYASLVPILLDHLTNSTHVKKEEIKYVCHRVVHGGMHDKGIRVVKGHEEGLMEMDKLSEFAPLHNHRAVLAVKSCIDALPHHTSLLLFDTIFHRTIAPEVYTYALPPPDTELTMPLRKYGFHGLSYASIVQSLAEHLKKPSDQINVVVAHLGSGSSSCCIKNGKSIDTSMGLTPLEGLLGGTRSGTIDPTAIFHHTEDAASDANVGDFTVSKAEIILNKNSGFKALAGTTNFGHIIQNLDPSKCSEEDHEKAKLTYAVFLDRLLNFVAQYLFKLLSEVPIESIDGLVFSGGIGEKGAELRRDVLKKLAWLGAEVDEEANNSNSGGAVKCITKEGSKLKGWVVETDEEGWMARMAKEEFGF | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 67673 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 80172 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 83055 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 36081 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 15825 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 55472 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06432 5' flanking region primer 1 | CGTCACACAACACTGCTACAG |
| L2 | CNAG_06432 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTTGATTGACGAGGAACCG |
| R1 | CNAG_06432 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCGAACTTAGTGGGTCTTGACG |
| R2 | CNAG_06432 3' flanking region primer 2 | GCGGTGATGGGTTGTTATC |
| SO | CNAG_06432 diagnostic screening primer, pairing with B79 | ACTTGGCGGTAGTCTGAAG |
| PO | CNAG_06432 Southern blot probe primer | ATACCTGGCGGCTAATCAG |
| STM | NAT#224 STM primer | AACCTTTAAATGGGTAGAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |