Detailed Locus Information for CNAG_06301T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | AGC/Akt protein kinase | ||||||||||||||||||
| Gene Symbol | SCH9 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNN00360 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
|
||||||||||||||||||
| Functional Annotation |
|
||||||||||||||||||
| Amino Acid Sequence | MLKGVKQLWPSSSGNNNNNNPSSTQSPSHSPSHSFSAFGGPPDQATPRASAHPQTDPFDAPQAVTSPAGLTKSAANLSLDPKRTSSPAGGQQPGLRTPVTHGFTSLPLATPGAGQPTTGVTVGDSVGASGGLGFGLHEPPKMRKAMANDGQGTTESTEAAMENSQTNLSHTSRTTGPKGTLRVKVISARGLAVSHSPGAEPQPYVVIQFEQNEYVSRPPHPVTSASSVPFTTSTPQPIGTPNNLTRSSSGLGVGAISRAFADAVGRGKKKEDGSGTMTPKAEEPAGGSWLGKPGPGDPVWKEEVSFDVTSSKPTLHLSVYDRNRDGEGFLGMLDIKPVLQDGYVLDNWYKLDTRGDETVTGEVWIQMTYTIIRKRISLKPSDFEFLKLIGRGTFGRVFQVRKKDTRRIYAMKVLSKKEIVAKKEVAHTIGERKILQCSLECPFLVGLKFSFQTDTELYFVTDYKCGGELFWHLQKEGRFSEDRARFYIAELVLALEHLHKYNIVYRDLKPENILLDATGHVALCDFGLSKPDLTDDKLTNTFCGTTEYLAPEVLLDEKGYGKHVDFWSLGVLLFEMCCGWSPFYAEQTQEMYRLICYGKIRFPKNVIGDDGKQFVKGLLNRNPQNRLGSHRGAVELKEHPFFKNIDWDLLYKKQITPPFKPIVDSDESVANFDPEFTNSSLLDAGIVPWEEVNANVDPGTASQPGKHSYLGPGAGISGSVPGGGGVAINKAQRPGLPGANGSPLTSSVQENFRGFTYTGESLMPQSMLADQSMDSDSDNENAVDDDEDDDEDEEEYEEDDDEENGIGSSRRECDVDMD | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 36517 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 25657 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 29673 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 15321 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 12126 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 25027 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06301 5' flanking region primer 1 | TTCTTCGTGCTGAGAGGAG |
| L2 | CNAG_06301 5' flanking region primer 2 | GCTCACTGGCCGTCGTTTTACAGATGTGGCGTAGTCAGCAC |
| R1 | CNAG_06301 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAATGAGAATGCGGTGGAC |
| R2 | CNAG_06301 3' flanking region primer 2 | GGATGGATGGATGCTCAT |
| SO | CNAG_06301 diagnostic screening primer, pairing with B79 | TTCTTCGTGCTGAGAGGAG |
| PO | CNAG_06301 Southern blot probe primer | AACCGAAACCCTCAGAACC |
| STM | NAT#169 STM primer | ACATCTATATCACTATCCCGAACC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|