Detailed Locus Information for CNAG_06001T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | phosphomevalonate kinase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | Ribosomal protein S5 domain 2-like | |||||||||
| Kinase Family | GHMP Kinase, N-terminal domain | |||||||||
| KEGG Linkout | KEGG::CNM00100 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MTTRTTTVVSSPGKVLIAGGYLVLDTHYSGLVIGTSSRFYSCVSSRATSGTSDIDGNTDTDTCQDEAIISVRAGQFPSDASTWVYSISKPSVSASASASASASASAGEDGGDRNPYLKLEQTNEEQAGKNKFIFITLCKVLEYVYESILAKVGDEETALDELIKRIKGGGFGEDGLEVVVFADNDFYSQREQLTSLSLPTRISSLPHLPPFTPLPRPIPATNKTGLGSSAALVTSLVGSLLSHLCITHVSPEGGDISDDDKAVIHSVAQLAHCQAQGKVGSGFDVSSAVYGSHLYTRFSPSILTPLMSLAPFSRPRPSTASTSLLDALHPSKWDNKSIPFRLPKHVRLLLADVSCGTDTPSFVSSVLKWRNNDREKADEVWERLDNANRALGEVLRDMVDAEGESDYEKTMMAAAELTSNELLNLHAAPSPSRTLHLLHRLALSLSSVRALLREMSDLSGVPIEPKEQTRLLDACGQVKGVVGGGVPGAGGYDALYLLTVDHPTPLAGVDKLWADWTEMDVCPLSAKQSDGGTRQEEVAEVKGLKEALDRARATG | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1232 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 3022 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3904 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 2804 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3456 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1342 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_06001 5' flanking region primer 1 | ATCTCCACCTCTTCGCCAACTTCC |
| L2 | CNAG_06001 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACCGTCATTTTTTTGGGATACGCC |
| R1 | CNAG_06001 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAAGAAGAAGTTGCGGAAGTC |
| R2 | CNAG_06001 3' flanking region primer 2 | GGAAGAAAGCGATTTACGG |
| SO | CNAG_06001 diagnostic screening primer, pairing with B79 | TTCCTTGCCCTTCCAATCC |
| PO | CNAG_06001 Southern blot probe primer | GGATAAAAGCCTGTCAGTCG |
| STM | NAT#119 STM primer | CTCCCCACATAAAGAGAGCTAAAC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |