Detailed Locus Information for CNAG_05965T0
| Species | Cryptococcus neoformans var. grubii H99 |
|---|---|
| Gene Annotation | hypothetical protein |
| Gene Symbol | IRK4 |
| Kinase Superfamily | NAD kinase/diacylglycerol kinase-like |
| Kinase Family | Diacylglycerol kinase-like |
| KEGG Linkout | KEGG::CNF00170 |
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. |
| Kinase Prediction | N/A |
| Functional Annotation | N/A |
| Amino Acid Sequence | MSGNQRQPLHIIVNPAAGQGKGPAFLDEHIIPILNHFQQSYRVYPTTAPLHAGEIGRSILDSRRELEKGGGPVVGGERDEPNVVIVVGGDGTAHELIEGVAIDQQAENEDQGWGRWELIVLPFGTANALYYSLFPPGDPLPDTSILSSLPYRPSPEVTAQILPLVSFLKRSSFPLPLPITLTTLLPALSQPLDVPSETSRNIKPIPTHVVLSTSLHAAILESSEKLREVHPGNERFRIAAQESMGIFFDARARLFGSSARVEGERRVKLGVKQWDPRVGSWVKPFTEHRSLADEDGKGESGIELQGPFAYFTSTATVSRFEPAFVISPLTSLRPSSFSEQSQQDGDGNGDGDGDGEGPRENKNEYIYIIVLRPLRDPYVLSVSSTSSRNDQCERPDRADQGQRWAKRAHEVLGKAYHNGAHIDLTYPLYAMEKKGSISGEGEEEEGWEAGVKGQGEPVVELYRCTAFSWEPTAISQTDRTNEEGSERARLVCADGAIHRIPEGGKAEVGLMVPSEKGGVYVWA |
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 1016 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 1180 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 1376 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1178 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1141 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 1082 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_05965 5' flanking region primer 1 | TCATAGACGATGTTGCCG |
| L2 | CNAG_05965 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACCAAGATGGAAGCCAGACTTAC |
| R1 | CNAG_05965 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCCATCTTCCTTCTCCGAAC |
| R2 | CNAG_05965 3' flanking region primer 2 | TTTCGGGAGAGTTTTGCG |
| SO | CNAG_05965 diagnostic screening primer, pairing with B79 | GCTGTTGTTTCTCACTGTAACC |
| PO | CNAG_05965 Southern blot probe primer | GATGTATCTGGCAAAGGGTC |
| STM | NAT#211 STM primer | GCGGTCGCTTTATAGCGATT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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