Detailed Locus Information for CNAG_05753T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | N-acetyl-gamma-glutamyl-phosphate reductase | |||||||||
| Gene Symbol | ARG5,6 | |||||||||
| Kinase Superfamily | Carbamate kinase-like | |||||||||
| Kinase Family | N-acetyl-l-glutamate kinase | |||||||||
| KEGG Linkout | KEGG::CNF02250 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MLRRSHIPRSALSINRAPAARAFTKPSLPLSASSQLTKKDSNNVLFEFDAHKVGNEIRKRGLANLLGVQREGGMDRDTIIRLLYSLGSRHEVERYLRIFTQSSRASPGGVLPEAKFAVLKIGGAILTNELEDLALSLSFLNRLGLFPVVLHGAGPQLNEILESEGVIPDYEDGIRITDAKTLAVARRVFLQENLKLTTALERLGTRARPIPTGVFTADYLDKSKYGLVGKITRVDKAPIEAAIKAGCLPILTSLAENAEGQILNVNADVAAGELARVLEPMKIVYLNEKGGLFHGVTGKKISTINLDEEYDALMKESWVKFGTKLKIREIKELLDTLPRTSSVAIISTDMLQKELFTDAGAGTLIRRGHKLYKQPSVEAVGSTQLRQVFTERDPEVESGRKSVAEIFSELKNSPHTIYGDEPFDVVAVVSHPAGETPVMTKFLPSRNGILNKIVDNVFDAIKKDHKRLFWTAKADDENRAWHFERADGSFTRAGRSLFWYGVSDVKEVERIIEGFEENGRIERVFLPVGPSIPPHRRAAAAPAAGATPAGARTFSTSARPSLIGASNTSARGYATVADAAPRKRVALIGARGYTGQNLISLIDNHPHLDLTHVSSRELAGLPLKDYKKSAVNYSNLSVEDVGMMAEANEVDAWVMALPNGVCKPFVDAIDRASKKGGKSVIVDLSADYRFENDWTYGLPELYGRDFVKQATRVSNPGCYATNTQLLLAPLMPHLDKVQMPAVFGVSGFSGAGTKSGEKDAEGRPKTVPKISAEDLKGGIRPYSLTDHIHEREASRHLSSLIPSSTNPFSLAFIPNVAPWFSGIVSVLTAPLDKSMRASEIIELYEEKYGKEPLIQVQKTVPDVTEVMGKHGWRVGGVQVHSSGKRVVVVGALDNLLKGAATQAMQNLNLALGYEELAGVPADKF | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 29223 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 23457 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 24995 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 26470 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 23758 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 19439 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_05753 5' flanking region primer 1 | ATTTTCCAGTCGTCCGTC |
| L2 | CNAG_05753 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTAATACTGAGGGCAGAGCG |
| R1 | CNAG_05753 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGATCCTTTGACCATCCAGGG |
| R2 | CNAG_05753 3' flanking region primer 2 | TTGATGTTTCGCAGCACC |
| SO | CNAG_05753 diagnostic screening primer, pairing with JOHE12579 | ACCAGTCAGCAACGAAACG |
| PO | CNAG_05753 Southern blot probe primer | CGACAGCAAGGGTTTTTG |
| STM | NAT#220 STM primer | CAGATCTCGAACGATACCCA |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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