Detailed Locus Information for CNAG_05274T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | STE/STE20/YSK protein kinase | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNH03560 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MPSPKQTEKLANLAVAYTLLEKLGAGNFGTVWKASHNDTKQIVAIKMIDLESSDDDISEIQAEIAHLSSCWSDHVTKYYGSFVRGARLWIVMEYLAGGSCLDLLKPGVFTEAQIAIVCRELLLGLEYLHTEGKIHRDIKAANVLLSATGDVKLADFGVAAQLSSHKSQRHTFVGTPFWMAPEVIRQAGYDSRADIWSLGITAIEMAKGDPPLSEYHPMRVLFLIPKARAPALDPEEGWSEEFQDFIEKCLQKDPHDRATAKQLLQHRFIRSAKKTTDLIPLITRYQSYKSSHPSSKPNAAPSKTLNRLAEGLGGLTIDGNGYGQGTMGSEWNFDETIRGTARGIPVNLNLEDMDEGFEDEDGHGSEDAWDAKVAEMEMWDGTVKENAGDLLVAGTSAGSEMSLPMFEKQPNKRNSTPPPGASASKTDPREEGEREQEDEPKTPTSASPSPSQRSLQSSSGSASSGKSTWKQRNDKVRGTVVKEGDVGDGFSTVKPMKKIDAAASQRLSMSYIGAGSVRRPNTNANTNINTNAHVFGPTPNSGPITESPTKSRPSVPLIADNFPRQTPQNQAQSQEKGANVEPEPRSLTGQALVEQVILPIISSTSSNPKHDLPAPTLESLSLLSKGFSDLSASSPELAYRLVMDILGGIRGNVAMGKEVGLGIGAGAQVELDGVDGVSGVRDEQRVDGEEVEEGEAKVKGDLVKERSAIADLLYLRWLDGLRSKGQRE | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 394 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 981 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 946 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 1389 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 2195 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 704 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_05274 5' flanking region primer 1 | ATGCTGTTTTGTGGGGGTAGGC |
| L2 | CNAG_05274 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGCTTCTCCGTTTGTTTCG |
| R1 | CNAG_05274 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTATCACAGGGCTTGACGGACTGAG |
| R2 | CNAG_05274 3' flanking region primer 2 | CACTTTTCTTTCTGTCCTCCC |
| SO | CNAG_05274 diagnostic screening primer, pairing with B79 | CAACAACGCCAAGAAAGC |
| PO | CNAG_05274 Southern blot probe primer | TTGGCGGAACGGATGAATCG |
| STM | NAT#58 STM primer | CGCAAAATCACTAGCCCTATAGCG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |