Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_05220T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationphosphatidylinositol 3-kinase
Gene SymbolTLK1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyPhoshoinositide 3-kinase (PI3K), catalytic domain
KEGG Linkout KEGG::CNL04090 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
PIKK179.63.2e-53
Microbial Kinome Result
PredictionScoreE-value
PI3K_Sugen94.22.6e-28
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K07203 KEGG 2.4e-245 816.3
Pfam Annotation
Pfam AccessionE-valueScore
PF02259 Pfam 1.1e-31 107.7
PF08771 Pfam 4.9e-19 64.3
Amino Acid SequenceMTVTSRLGPLIHMDLFQMAFVNCYMQLQDDAAFKMVVDETIISLLSDKAVSKEILITFLDLLAFCTKDTTPYFSPKVFEVAKMCALQCFHGGLNQKLPGVVLWYIEQQAEALPTAESISNLVEANIRVGSGGYDAAWSTLLWLENDWSIEPQPMWITSLSHWQQALSAQDKIDKNQQATMYSSFNLRMICYHALGDYQKGYDLAQNFFEGLDDNERRNTAHWATAAAWHMGDFDTMADHLAFHPRGASKSLYKAIIDIHNEQYASAFHHINKAHSLSYDELQMQLEAGLQVAQKSLAKTEFLVELQEAIKYKSQPELRANILATWKTRFKRSHADANSWLKRLEIWTLACSPKTFELQNCFLNTAKLCESAGMHEAARSIIKRITPDVTPPGCKVEYTKLRFQWKDVFQKHDQNGMEDVLECLYIHTRRYLEYINVNKDELEASALGLQPLSKTEDLSTLERKIIARRYYRLGEWTSVVQGSEWLQDRDSTVLTYTSLAAKLDVCWYGAAFSLAERSVTLFESNGFSTSDGAAVGSYTLRGLFQAARTKESPEFVIKALLRLVTLWFRFGENQAVLVEVENQLHITDVEPWLSAIPQLIARLGTPQKDLQCTLIKLLKTISSHYPHAVIWPLLTATQTRKVEHQEAARVIMDYICTMADGARLVDQAELVGRELIRTSISWLEKWKGIIDRFLPRQDLMEIKWQDIPDIWEEDLKMLKNPETPDEHQFVLQYGDQLHLVDKTLIRYNSSRQLNLVNNAYSALYRLYGEIEAQLNQWRLPGMKLHLASTAPRLLSLRDCILTVPGLYDPHIKLDDQAFIDGFQPAVHILSSKQLPRKLVIQSYLSDHTFLLKGNEDLRGDERIMQLFNLINTLLNHHSDAFGRNLHLLPYEVVPLSPSAGLVSWVSNTQQLQSMIQANRDKHKRNALTNQELGSLLGYDPDAFNPNNPKLDAPAEMDKYDKLPLPIKVQRLKAALSHSNQSDIKDVLWQRSPSADIWIRRRTNFARTVGVANFVGYILGLGDRHGSNILVDQLTWGALHIDFGDLFNVAQERSFLPEKVPFRLTRMMTNAFELASQGDLETPGSTGTFKQASLITMDVLRDSRSTLLAMLEAFLYDPLLSWTSSQNNPSGATKLEEAVRKAPKAGASLEELKSKIGLKDSSESTQSQQSKVKDLSIPEDSLHYGQLDNSLTSTCLRTDSYMARVPSTEMTNSKALQVLTQIERKLVGLHNTNSEEPLSVSKQVQELIKEATNLQNLSQGYVLGWIPHW
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 1987
B YPD 30°C, concentration: 5x10^7 cells/ml 527
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 564
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 514
E YPD 37°C, concentration: 5x10^7 cells/ml 578
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 937
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_05220 5' flanking region primer 1 ATCGCTTCTCGTTTGACC
L2CNAG_05220 5' flanking region primer 2TCACTGGCCGTCGTTTTACATCAACGACCATCTGGGAC
R1CNAG_05220 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGTGGCTACTGCTGTGTATTGC
R2CNAG_05220 3' flanking region primer 2GCGGTAAAGGTGGAAAGTC
SOCNAG_05220 diagnostic screening primer, pairing with B79CTTTGAAACCGACCATAGG
POCNAG_05220 Southern blot probe primerGGACCGAGACACTACTCACAAC
STMNAT#116 STM primerGCACCCAAGAGCTCCATCTC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_05220T0
Mating Data
Growth phenotype for CNAG_05220T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_05220T0
Urease
Urease phenotype for CNAG_05220T0
Melanin
Melanin phenotype for CNAG_05220T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_05220T0
Oxidative Stress
Oxidative stress phenotype for CNAG_05220T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_05220T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_05220T0
ER Stress
ER stress phenotype for CNAG_05220T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_05220T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_05220T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_05220T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_05220T0