Detailed Locus Information for CNAG_05063T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | STE/STE11/SSK protein kinase | ||||||||||||||||||
| Gene Symbol | SSK2 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNL05560 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MSNPTSPSNPSDTGPSSASNVTSSSSKTGRRSVRLFAPDEEDSSDEDGGLIGVPAETTFKDDEIPPSNPRSASYPGPPAHTSPTSKISTIVSSASAAQPKLARSITYVAPNAVSSRPAYPLNPAGSETLHASYETWRKTRYTLESFGRGSKPDGKNTAQRGRSYTDPDIGYFSHDAGDDGWGSDDDDELRSPGWGISHHNMDSGGKTNGSPQLPIKPADVTEDEGQERLDWQGMLESVLNSDVLKVEEQRIYNSMPTDSFREEIGKTLWWQIRAKLRGRTEAEEKKRVQERRARVVDPVLEEINEFKYDPKNNPEGEEDSDGDPQDATSTAAPQSKALNQVNTVLAKLHAIKGLYPNLAAMRADKVLYTDENFRKRADALTSWSIIVSSLQTQLKLLQKWTGSDELDITKPNTTHEKALVGKYKYHSIDSKGTPGRDAADDSSFLDRVIKEDNLQRTFERRAFVDMINLVRNAKETVISYLPQFQEQNLPDFQYEIVRLIGFPGRLIIEAVKVRLDAASRLLDPNPMVVEDFIENLRLSISLAVLIRKQYDEIMAPDAEGRWKIPHCLPTEYNDVLLDALRTFFKLLHWRLRGVGKASYYKETEVLEEEAPFLYEAAEAIVGGDMVVAEQYCALSNKLLIRSANYLDQQLRVPIHSPSRDKERGDKERDGSSSSQRNRDGRDSSLPGPPKHMKVEELFSWYSKLLDSARMRHRKTQRFCRKLTQRFDNSAEYSIEETEMDMLVETLQDTGHFLVYTGKFEANGTYIVADGSLWGQPDDVRHLLKRVFSVTIPGSRVRPRQTTSQVSVGGASPSNGQVAAQHDPADPYPEADDFDDEALAAYILLISPRQSFVWSGAVMTLDVDYIDYELPDNRVRLIADGPTKRLALCKLYFKQALIHPDTGETIDLPCVVEAQAHLPTIQKQLVKIAKSSYRLSECIVQSAPLVRNAFRGKPGSQELVENWYSFATEHGTRVLIHIEPSVWERFNRLLMRLAISWISFISQECNPTDRKTFRWTVAALTYAFNMTRGSNILALDRSEFSLLRRYVGVCVSLLVSHFDILGARSSMEAKKEADRIEAMRRLQRLQENLDDEFLPRTPTESGDQPRIDRSIRLTVEERLRLIAELEARRDELAPAPVGQVLDEEVSEDRALVFLAASKSNISMRWQQGAYIGGGASGSVYLGYSLQDNTVFAVKILPTVDLQSSPALYESIKRESDVMSLLSHPNIVGFLGLEVHRNRVCLFQEYCEGGSLAGMLEYGKIDDEEVVGAFTIQLLRGLEYLHTNRIEHRDLKPENILIGANSVLKLADFGTAKIIKSNKTLARTRGGAHAKMEGLEGTPMYMAPEMIKNQRTGKLGACDIWGLGCIVLQMITGRKPWSFLDFDNEWAIMFHLGATKEPPPLPDPNEMSDQGIEFIDQCLSLDPEARPVASELLQDEWLVPMLEQMAELEQEYPDILAMGQSDSFAPPPENASLTSNAPIFQDDVTPPPLE | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 5215 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4380 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 4426 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4083 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3643 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 6387 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_05063 5' flanking region primer 1 | CCTATCTTATTTTTGCGGGG |
| L2 | CNAG_05063 5' flanking region primer 2 | CTGGCCGTCGTTTTACTCCTCTTTGTGCCGTATTC |
| R1 | CNAG_05063 5' flanking region primer 2 | GTCATAGCTGTTTCCTGATGTTGGAGCAGATGGTG |
| R2 | CNAG_05063 3' flanking region primer 2 | CGACTCGTCAACCAAGTTAC |
| SO | CNAG_5063 diagnostic screening primer, pairing with B79 | CTAAGGATAGGATGTGGAAGG |
| PO1 | CNAG_05063 Southern blot probe primer 1 | AAGGACGACGAGAGTGAGTAG |
| PO2 | CNAG_05063 Southern blot probe primer 2 | TCCAAACGAACCTTGACAG |
| STM | NAT#210 STM primer | CTAGAGCCCGCCACAACGCT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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