Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_04577T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationnucleoside-diphosphate kinase
Gene SymbolUnknown
Kinase SuperfamilyNucleoside diphosphate kinase, NDK
Kinase FamilyNucleoside diphosphate kinase, NDK
KEGG Linkout KEGG::CNJ00180 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K00940 KEGG 2.1e-68 229.3
Pfam Annotation
Pfam AccessionE-valueScore
PF00334 Pfam 3.9e-52 174.2
Amino Acid SequenceMFANSLRHGLRTASRSSARAFSTIPARAPRTNVVGALALGTAVTGYALYESTRSPVLLEGPRTIAGEKGTVTERSFVMIKPDGVSRQLVGKIVSRFEERGYKLVAIKSLTPSDALAKEHYADLSARPFYPSLVKYITSGTPVVAMVWEGKDVIRQGRRIVGATNPLDADAGSVRGQYAVSVGRNLIHASDAFESATKEIGLWFAPEELSEYEPIAWPWVMADN
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 4316
B YPD 30°C, concentration: 5x10^7 cells/ml 6801
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 5928
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 6570
E YPD 37°C, concentration: 5x10^7 cells/ml 5920
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 3638
Gene Disruption Strategy
Primer NameDescriptionPrimer Sequence
L1CNAG_04577 5' flanking region primer 1AGGTTTGAGCCATCTGAAC
L2CNAG_04577 5' flanking region primer 2TCACTGGCCGTCGTTTTACAAAGGGCATAACCAGTGAC
R1CNAG_04577 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGGTTGGAGTATGGGAGATGC
R2CNAG_04577 3' flanking region primer 2GTCTTTTCTTTCCCACTTGG
SOCNAG_04577 diagnostic screening primer, pairing with B79AAAGGGTAACAACCGCCTC
POCNAG_04577 Southern blot probe primerGCTTGTAACCACGCTCTATC
STMNAT#282 STM primerTCTCTATAGCAAAACCAATC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG