Detailed Locus Information for CNAG_04335T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | phosphatidylinositol 4-kinase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | ARM repeat | |||||||||
| Kinase Family | Phoshoinositide 3-kinase (PI3K) helical domain | |||||||||
| KEGG Linkout | KEGG::CNI02140 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MDSLDEPLHLHILTSLASSYAAHPSNSDEQPINALTVGLPQTALSTSAGLDLLGSDEEDDEWTELEINHAISYAHYVYSLPITSANLASSVGKLKKLLWGLIRQGTIVVDPAASPASSSSARANVGLAGSVGQAQLCQDLIKAMIWAGWSSEDARSDVGETLVRLGQQIADLCASNAQTTFPLVLLNSVHSVITHCPLPPFPTSIIARLVSTFLPISSPKFLVKLVRQSSSAASTPHFRLPKNSPFLAQYPSSPVAVVMLVTEAITALLGNSLFSQPDIDEVQKAFVKHSDQMNSSFLPNPEPPGSRPVLEHQISQLVSFPVEGHSLSLTSEGRAVLAEVDRMSLRWWSDLMGSAYIDTERELPGKRGNLFAHGRNEPEEEVDLTVAVLHLMNMIVLRQPDGDKTQLARLKMLLSESSTITDARVLEAAFTCTTILVRNNPSLGTPMTHHIRRLLMTPLPAFEGEIAGLGGDVPPAVVAAGKCLSVCIEMSADDDLTSSTLYSLLNTLSHGQGNSGSAGVNSIRSLPLHHQGGEADTYTLKTTLTGGKGSEEQKRLVAAAAVQVVSRLALELGKDDILHLSLAMLLQRLRGVDITTEATIVANLVPLALASSNADLVEVYRAFSQIARSSHPEDPRRSSNAILAALTTLAKGMYKRLDCADGYLVELLTLFADKGTQTQMISMAAHAYDSKDREQLAQLRTEGNTRVAEMKSWLSALLIPISTLLSHPHYHPDEAASPELVTHFRNLWFTLVVFGLSGNVGKNNLNEHEWEALGVIAEKTPVLVLESSNDFVESELEYNSTLRKDFAASIQHRQSTALNEYLPQQRQSYDTRNLSTPQITFLMAVHDLEEMRTLRHRPSVVLQYFCNVSLNTSSLIGCLDAIVQKVSKSFLRQLYHQAITHSLPPIISKEVCKILIACTHRMSKVREKALAYARLILETFSALLCDKEVVFTLLEILTLMRRACETQYTDEYSPVYEFSSDRMDLTLHLTDDYAVRNEIITQLHTVTCTWLTLAISRAPIEVQSILQSYLNEARDVLLIDSVEMGAGLALHFSKAISRLDRQETIMPNIGGWPSDSSNLVASQFASKNYHTGELSGARLVLSYGLSDLQKGSPTESSQAELVAFKSQMAQAVSNIRINSKSLDVPEIRRLLLRGVSVLVASRRMDRDILHYLVELPMVAFTPLAIAAGVDAWTWLLRQRPEAEVAIVGAITAGWMQTIIEKKGFFNNSMNYQDPFERPVEYSPSDKKVTDLELAKARRLLRPHTLLVQVLSSQFHAIKYRERAMTISLIRLMMRSLEAHREMSTHPLAREVRFSFLLLGFQILSSSRMEALLELRLRDNLYKAAFSWFSVRPQWSYGSDRIQVGAEIKLLQDFLSAVANDGIRAEHYTSSMADRTPAFLIPGASSIQEYLVQHKDRVRLLQLLVENEISRLAVWSNPVNEHGRTSAPAVGSLERSTSTADWTRLLQMAWKLNPAMAVQMGERFKIAPLQAEITHLVKSDPKAVQDVAEALHFLLGDKLEQDAKAALKWLPVWAAVPPVTAIVYFQPRYGNHPLILQYAMRVLEQHPVELTFFFVPQVVQALRADGLGYVERFIFETSKISQLFCHQIIWNMKANTYRDDDASQPDPMKPLLDRMIDMIVHGLSGKAKAFYDLEFDFFDEVTSISGKLKPFIKKSKPEKKAKIDEEMAKIKLSVGVYLPSNPDGVVVDLDRKSGRPLQSHAKAPFMATFKVQKEKLDLPSNQSIENADEAKTVKTKYDVWQAAIFKVGDDCRQDVLALQIIAMFKNVFTSIGLTLYLYPYRVTATAPGCGVIDVVPNATSRDEMGRAKINDLFSYFVDKYGGVDTASFQKARLNFIQSMAAYSVACYILQIKDRHNGNIMIDGEGHIVHIDFGFLFDIGPGGMKFEPSSFKLNHEMVALMGGRDSQGYKMFTELTVKAFLAIRPHADQLIDAVHLMLGTALPSFKGEGTITRLRNRFQLQLGERQAAEFMMGVIRNAHENMRSNVYDGFQKMQNGIPY | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 10749 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 9024 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 10675 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 6593 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 5765 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 9011 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04335 5' flanking region primer 1 | CGATAGAGTAGTAGTTTTAGGGGG |
| L2 | CNAG_04335 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACCTTACGAGTCCATCTTCGC |
| R1 | CNAG_04335 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAACCGATTCCAGTTACAGC |
| R2 | CNAG_04335 3' flanking region primer 2 | AGATGGACGAGGTGGTGATG |
| SO | CNAG_04335 diagnostic screening primer, pairing with B79 | TGATGTGCTACTGGAAGCC |
| PO | CNAG_04335 Southern blot probe primer | TCATCAATGTCAGGCTGGG |
| STM | NAT#146 STM primer | ACTAGCCCCCCCTCACCACCT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |