Detailed Locus Information for CNAG_04314T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | NAD kinase | |||||||||
| Gene Symbol | YEF1 | |||||||||
| Kinase Superfamily | NAD kinase/diacylglycerol kinase-like | |||||||||
| Kinase Family | NAD kinase-like | |||||||||
| KEGG Linkout | KEGG::CNI02350 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSHNRSDKAFESFRPMREQASSVAPAASSPNPNHVSQCFVHSLFKKRCLAALSSDESQSKKTPENQTPYYEDEDEENESLTKQLAETAQSVREMNKELGRRIVRSRIQHVLIVTKVNDNQLVSLTRELALHLMQKSPSIPKGTNSRYGTASLGRGMVVYVDSEFETSKAFDVPGLQRDHPELFEPMISNRSSSSLSSMNTLNESSTDEEPVTSGSSTPVGEGQLRYWTNKLCSNTPHLFDLVITLGGDGTVLYTSWLFQRIVPPVLPFALGSLGFLTKFDFKDYKEIIDKVIRDGIRVSLRMRFCCTVYRTSTPGNIDGPKAKKRRIIKDGSASALKKRVHKSGWESLEDEEADSHLSDAGSDEDVIMRHSTRPEEQFEVLNELVVDRGPNSAMSSLELFGDEYHLTTVQADGLTVSTPTGSTAYSLSAGGSLTSPQTSNILITPICPHTLSFRPVVLEDSIDIRVCVPFDSRTTAWTSFDGRSRLELKQGDHIKVTASRYPFPIILYADKSFPDWASSLSRKLRWNERERQKPYVLVEEARQQK | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 57471 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 24114 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 23960 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 15374 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 8178 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 45725 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04314 5' flanking region primer 1 | CCATTCGTAGCCCTTATCTG |
| L2 | CNAG_04314 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACACGGAGTCTGGTTTTCAGG |
| R1 | CNAG_04314 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTTTGATGGAAGGAGTCGC |
| R2 | CNAG_04314 3' flanking region primer 2 | AAGAGGGCATCACTAAGGC |
| SO | CNAG_04314 diagnostic screening primer, pairing with B79 | ATTGGACTGGACCATAGCC |
| PO1 | CNAG_04314 Southern blot probe primer 1 | AACACCAAACCACGGAAC |
| PO2 | CNAG_04314 Southern blot probe primer 2 | TGGCTCCTCGTCTGTAGAAG |
| STM | NAT#224 STM primer | AACCTTTAAATGGGTAGAG |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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