Detailed Locus Information for CNAG_04191T0
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | SCYL protein kinase | |||||||||
| Gene Symbol | CEX1 | |||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | |||||||||
| Kinase Family | Protein kinases, catalytic subunit | |||||||||
| KEGG Linkout | KEGG::CNI03290 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
|
|||||||||
| Amino Acid Sequence | MNYLKSITTSVLQSTGVTFPFSVGERIPGLDSHSSIWEIREGVKRDDGTPLTLFIYDSTLPPFQPGSRDRKVLFQLAKNALKKLRTIRHPDIIRYIDSVETETHIYIATERVRPLEGVLRDWETGGALASGAGRAKGKENWIGWGVRSVSSALAFLNSPPLSQHHSYLIPTSIFVTPSLEWRLAGFELLTGKDDAGGILWGLGGVAPGDIGERCAPEVRKGGWSALRETDPAQHDTYLFAVLLFTLYNPHSPLPPLSSQPTPSSSGTLPKVLFPLWKRMLNPNPRTRLTTISLTDEAEKAGFWASNPFVSLVKGLDNFELQSESDKLSLLRIIKEATTQAGTLPGPFTTYKILPSLLHSLSLPTAPSSAMLPLVLELSKLVPSSDYPKMVLDPVVRLYTSPDRGTRMALLEGLNEYVDKLDNKTVTEKIWPNLITGFADTVPVIREATIKAVYPLASKLSDRILNNDLLRVLAKMQMDTEPSIRTNTCILLGRLAPILGHNTKKKVLVPAFARSLKDPFVHARVAGLMALMASVECFDREDLAGKVVPNMSFTLVDKEKLVRDQAFKAMNMFVSKLTDMVKDMPETALPSDQISSQGPVTTTSASISSNQGGAVNSAAGAAGALAGWAISSLGKQLKTSEAHSSMNSSGMPAGFGASAPTIKFNTASSSPAVPASSRVSEEIFRPVSASPSLKSVSPQPRAVNAFGRTTTNSSLSGTSGGMKLAGAKKAPGKSLADQLAGEWEEEDGGNAWGTDDLIDVNADDDDWSAFESAPVPEVVVPPPQPYYVSAPKPKPSSSNITTSAALRSKPTLSPAISSTSSIPTHAPEPVKPASSTSTPLIPSSPALSTISGAKSPVLSSQVNTTDTERGQMDSASASERSSPQLSVKAGNGTGTLQPNLANMSKEEKEKEMARRREERKAKIAAMKEQKKSKA | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 4044 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 5788 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 6075 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 4483 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 5112 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3823 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04191 5' flanking region primer 1 | CAAGTGGTGTCGCATTTC |
| L2 | CNAG_04191 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACCGCAACCTGTTTAGTCAGAC |
| R1 | CNAG_04191 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGCAAAAGAAGAGCAAGGC |
| R2 | CNAG_04191 3' flanking region primer 2 | GGGCTAAGAAGTTTGATGTTCC |
| SO | CNAG_04191 diagnostic screening primer, pairing with B79 | ATGAGGGTTTTCAGCACC |
| PO1 | CNAG_04191 Southern blot probe primer 1 | GTAGGGTTGACAAAGTGGAG |
| PO2 | CNAG_04191 Southern blot probe primer 2 | CCCTCAAAACCCCTTCTAAAG |
| STM | NAT#219 STM primer | CCCTAAAACCCTACAGCAAT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|