Detailed Locus Information for CNAG_04083T1
| Species | Cryptococcus neoformans var. grubii H99 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | dolichol kinase | |||||||||
| Gene Symbol | Unknown | |||||||||
| Kinase Superfamily | Not defined | |||||||||
| Kinase Family | Not defined | |||||||||
| KEGG Linkout | KEGG::CNB05650 | |||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | |||||||||
| Kinase Prediction | N/A | |||||||||
| Functional Annotation |
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| Amino Acid Sequence | MAVQSSRSHHHRVRPRSHSRSSSSPSPPPAPLQHNYIHPHSSLRGSQSSLHSARDIREKRKSFNAHRTSMLSPGGHRSRALSRGAIPAFPNMDDRQPNPGDENVLVVDTDGGGFSTQYGASASSMTGVSGSEQEFLTGLRDETETEVERDKRDIAQWRRSVDDLIDRAPTPLALNNPRHTISSRRPIPQPAPPFTLLPFRVRFPCLSAPISFGWTAQRRIWNLPIKMTRRRCENALVLGAVLWGIWVLVVKWDEKAVAGEIGLLVGLTLLYTALRFQPTPQRLLSQPPPTNRPTSPVVASPSSFSSSYARERIRRSSMSNVNGSLSPSFPKDDLRGRAFSPDEDGRMGIGERASIWGTEEREYRECPDDGIFYSLLLGPLVAAALLHSALSQLSSNPSSPIANGWNIETPLVLPSTPIRRGANITDEEPTNTIRALSALATSRRNLVQLFTLCSFVLLVHVTRSLHLEITQFRISYPSNAITPDAEIAKLHSLQAQQASIGTYWLRKGEWKRTGSVVGFSFLVTGVCLLVKAVTAKVGRGVWSDMSPSDIVIATLFYQFSIYVCVRLARRGVTLGELAAVCNAATAMVMETINLTRMKISIFRTPYIKTYRLPTPLLIFQLALIPGSLLAGFLLSPLLYLSRNLAQKPAHRLRLPHEKPVHRRLLALGFYAGSVLICGGLVGAWTKWCLGGRNPFTWSFFFIFEGTHAWTRPALIAYWVALAVISVAGWERQLNRARKHRRYVVPGTAAIRGEGPGGPHSVAGGPTGKQLGLGSGYAFANATNDGQPGGAAQEGFTGVATQMMDAADQRMPTLSVNARRKSFHALAVIMFIPGIAVDPAFTHLSFSVAFAAFNFAEYVRYFALWPFGVKVHLFLNEFLDHKDSGTAILSHFYLLAGCAAPLWFEGSSDILSYFGVLSLGIGDALASIVGRRIGRLRWCIVFGKTVEGSIAFFLSVLGVSWIMWTFGIVDDFNFKPYAITVGLSTLLEAFSAQNDNLILPMFGWAVGTLLGV | |||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 11540 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 7011 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 8123 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 5509 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3600 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 9126 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04083 5' flanking region primer 1 | TTCCTCCATCTTCGCATC |
| L2 | CNAG_04083 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTCGTGCCCTTTTTGGTAG |
| R1 | CNAG_04083 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGAAAGAAAGAACACCCCTCC |
| R2 | CNAG_04083 3' flanking region primer 2 | AACAGGTTGCGATTGTGC |
| SO | CNAG_04083 diagnostic screening primer, pairing with B79 | GCCGTTATGGGTGAAAGAG |
| PO | CNAG_04083 Southern blot probe primer | GAAAGGGAGAAGAGTGAAGG |
| STM | NAT#210 STM primer | CTAGAGCCCGCCACAACGCT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |