Detailed Locus Information for CNAG_04040T1
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
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| Gene Annotation | AGC/RSK protein kinase | ||||||||||||||||||
| Gene Symbol | FPK1 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNB05230 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
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| Functional Annotation |
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| Amino Acid Sequence | MSSLASPPPTASISTSDSYGSPIQSSGPGLSSGSSTPSKWKSVFKLGRTATIGKGKENRSALGGESFVLDNKEQQSFAGGVSPSVVTEPYFPPRGTQLRAHTDPYPPKSRSVKNNLGSASVEHFDSKVLHEEGMSKTREEGSSESFNIHLNSSNGNLQTTSTSGEQSRPYSSVTDSASASTTERTSHSSGGHFPPADGSSHGMAASNAYGTTSTRSPSGGPLGIGSFKSRFFSAPLSAPAGKNKHDTSKSERYRGLGKVATDLPQSTAPPSSAGGASSNGGGRKKSNGSSSFSSRGAASPRTPAKSKREPSANSSKRIPFGQTNENGPNSGSAAARFLRRVVSAPNTKALFSNNLFSNAPDVPPLPTSKNQPNSSTSSKEKPSSPVVVVGNSEGHIDLTSSPDSYRPPSISPLSNSSTMYTSQTSHTPPASASATPTPSPLKQKNHFLSPTASPSPGLGSGLSARGTRANRSLTTGTAPVLKNIRQLQGYSGLSPGGGGYMDGESRHKQVFRRTYSSNSIKTKQVEVTASSFQKIKLLGKGDVGKVYLVREKKTDKLFAMKVLSKKEMIKRNKIKRALAEQEILATANHPFIVTLFHSFQSQDYLFFVLDYCMGGEFFRALQTRPGKCLSEEHAKFYAAEVTAALEYLHLNGYIYRDLKPENILLHQSGHIMLSDFDLSKQSGEAGGAPAAIRHGGPNGQTILVDTRSCIADFRTNSFVGTEEYIAPEVIKGHSHSSAVDWWTLGILVYEMIFATTPFKGPNRNATFANVMKNEVLFPETVPVSSNCKSCIRKLLIKDENKRLGSASGASEVKQHKWFASVNWGLLRNMTPPIIPEESNGIDTINFRPLRESKSIDFDRDDITTDIIHAKAGSPSVSGNATPGMLTPKELVQTTSSPSPSGGGAGGVSGHSSSAMPIPGSVRGYDKEKNPFGEFSSVTRDFGEC | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 5139 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 3947 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 3862 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 3003 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 3496 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 6184 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_04040 5' flanking region primer 1 | ATCGTCTCAGCCTCAACAG |
| L2 | CNAG_04040 5' flanking region primer 2 | TCACTGGCCGTCGTTTTAC TCTTCCACTTTGACGGTG |
| R1 | CNAG_04040 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTCCGTTTGGGGAGTTTAG |
| R2 | CNAG_04040 3' flanking region primer 2 | GGCTATCTTCTTGGCTTGC |
| SO | CNAG_04040 diagnostic screening primer, pairing with B79 | CCTTTGGGTTTTTGGGAC |
| PO | CNAG_04040 Southern blot probe primer | ATTAGTCTGCCCAAACGG |
| STM | NAT#211 STM primer | GCGGTCGCTTTATAGCGATT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
| OEL2 | CNAG_04040 5' flaking region for overexpression construction | CACTCGAATCCTGCATGCGGGATGTTTGTGTGACTGAG |
| OER1 | CNAG_04040 5' coding region for overexpression construction | CCACAACACATCTATCACATGTCGTCTCTCGCGTCACC |
| NP1 | CNAG_04040 Northern blot probe primer | TTCAAACTCGGGAGGACAG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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