Detailed Locus Information for CNAG_03946T1
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | galactokinase | ||||||||||||||||||
| Gene Symbol | GAL101 | ||||||||||||||||||
| Kinase Superfamily | Ribosomal protein S5 domain 2-like | ||||||||||||||||||
| Kinase Family | GHMP Kinase, N-terminal domain | ||||||||||||||||||
| KEGG Linkout | KEGG::CNB04330 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction | N/A | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSAKVPITVFNSLQEIYPSASAVLREGQRWNTLLARFQEQFGEAPAYIVRAPGRVNVLGEHIDYSLFPVLPAAIEQDILFAFCPTRPVAGSNPTVRLENYDRKYSYPGCSFSLVPGEKGWDVGLDARGGWDKYVRAALLECLDELFPVGKGDGRQEAVGMDVLVSGSIPPGSGLSSSAAMVVGSVIMFLVANNLAAGKTKADVVQLAINSEHRMGLRTGGMDQSASALALPNNLLHLSFHPGLLPAPLPLPGNVSLVITNSLAPHSLTGSAPEEYNLRVIEILIATRLILHHWKLESHFNDNPRPWLREVLGAWVGEKGHMGWEKEGEVMEKALGDIEWIKRDGGWSREEMVKYSGMNEEEFKKSYLDFLEIRAEKFHLYERLHHTLTESLRVHKFQPTSSASSASYSMHLTLPCEIRMTAPTLSWIRCKSCV | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 4698 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 4776 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 4785 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 2828 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 1723 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 3599 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03946 5' flanking region primer 1 | AAAACTCACATCCGCTGC |
| L2 | CNAG_03946 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACGCAGAGAGTTGAAGACGGTG |
| R1 | CNAG_03946 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGGCTGGAGGTGAGTTCTGTAATC |
| R2 | CNAG_03946 3' flanking region primer 2 | CCCTATTCCTTTCCTTGTTC |
| SO | CNAG_03946 diagnostic screening primer, pairing with B79 | AGACCAATGTAGACCCTATGTG |
| PO | CNAG_03946 Southern blot probe primer | ACAAGCACATCCATTCCTAC |
| STM | NAT#218 STM primer | CTCCACATCCATCGCTCCAA |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
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| Mating Data | |
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| Virulence Factor Production | |
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Capsule
Urease
Melanin
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| Stress Response and Adaptation | |
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Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
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| Antifungal Drug Susceptibility | |
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| Virulence Data by the Insect Host Model (Galleria mellonella) | |
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| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
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