Detailed Locus Information for CNAG_03796T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | NAK protein kinase | ||||||||||||||||||
| Gene Symbol | ENV7 | ||||||||||||||||||
| Kinase Superfamily | Protein kinase-like (PK-like) | ||||||||||||||||||
| Kinase Family | Protein kinases, catalytic subunit | ||||||||||||||||||
| KEGG Linkout | KEGG::CNB02900 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction |
|
||||||||||||||||||
| Functional Annotation |
|
||||||||||||||||||
| Amino Acid Sequence | MSRIPDTMDSDAAAIALDRLKFYARDALLTITQCICKPDATLKINGRSYKIEKLLGEGGFSFVYLIRDLSSDRLYALKKILVTSGQEGVKEAMREVEAYRRFRHPNIIRILDSAVVQDESGDGKIIYLFLPYYSKGNLQDAMANASVTGQRIPERKLLELFHGTCLAVRAMHQYHLPNISASYPPTREDEPLVGETVFDHDEELTEEDQGELVPYAHRDIKPANIMISDEDEPILMDFGSTIKARIAIETRQQALLEQDIAGEHSSMPYRAPELFDVKTGKTLDEKCDIWSLGCTLYAVAYGHSPFEVDGQSIAMAVGSGRYRHLTGYSQDFVQLIDSMLIVDPENRPDIQKVIEMTENILRKT | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 3640 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 5383 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 5662 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 5242 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 4088 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 2817 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03796 5' flanking region primer 1 | AGGTCGGAAGATTTTGCG |
| L2 | CNAG_03796 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTAGGGTCGTTTGTGTTATCC |
| R1 | CNAG_03796 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGCTTTTGGCTTTGGGTCAG |
| R2 | CNAG_03796 3' flanking region primer 2 | TGAGCAGTAGTGTATTGGGTG |
| SO | CNAG_03796 diagnostic screening primer, pairing with B79 | AATCTCCTCTTGGGCTCAG |
| PO1 | CNAG_03796 Southern blot probe primer 1 | AAAAGAGACATCGGCTACG |
| PO2 | CNAG_03796 Southern blot probe primer 2 | ATTTGGATGCCGAAAGCG |
| STM | NAT#227 STM primer | TCGTGGTTTAGAGGGAGCGC |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |
Phenotype Assays
| Growth | |
|---|---|
|
|
| Mating Data | |
|
|
| Virulence Factor Production | |
|
Capsule
Urease
Melanin
|
|
| Stress Response and Adaptation | |
|
Osmotic Stress
Oxidative Stress
Heavy Metal Stress
Cell Wall Stress
ER Stress
Genotoxic Stress
|
|
| Antifungal Drug Susceptibility | |
|
|
| Virulence Data by the Insect Host Model (Galleria mellonella) | |
|
|
| Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model | |
|