Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03791T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationCAMKK/ELM protein kinase
Gene SymbolUnknown
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNB02850 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Kinomer Result
PredictionScoreE-value
AGC50.75e-21
Microbial Kinome Result
PredictionScoreE-value
ePK204.75.1e-61
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K21157 KEGG 1.5e-190 634.6
Amino Acid SequenceMSAVPQPAGSLPDQRYSFKSPATLQPGPSDCPESLRRTASHTSQASAQVPSPRPTARSFSKGQFTPRILSRRGSAQTGTEYSFKALSRTSSAREASVHEDGWRSPSIDSTPAEVIETQAVRVKRDHESGKWMINQYRVLSEIGSGTHGRVRLGQDMTAEIPAADDDGELHPPTCPENSFYAIKIVDRNPKRKRLTGLGRHKGSSGGAKLLNENEIRKEIAIFKKVNHPNVVRMKEIIDDPESSKIYMILEWCQNGEIKWKDGEGLPALTVGETRKIFRDTLLGLEYLHHQGIIHRDIKPSNLLRSGDNTVKISDFGCSHFSEALRAAAAQPGPEGDAYVDDIELAKTAGSPAFFAPEMCYSGLDSEGSSRPPSQPSTNQEVPSFIIRPPSSGDTSVDTSSSLMSLPRSSTTFPLKPTDSNDSAGSRRPPSFRSQSSSATIQRRERLPITNAIDVWALGVTLYCLLFGKTPFNAPNEYLLMQVIVSESYIVPPFMGKDRLPTGTGGLPAAEEAVECLDLLKKLLEKDAGKRITLEQAKQHGFTLHGLADPAAWLAKTDPHTHSFVTVSNDEVAAVITKSVRFRDRFRKGIKTISQKLQLLGSGRTRSHSFGDGESMGTNTEYSLTPAGSTSQCGTPKSNKLSALLPVSTPNKDVSPMNSPLPYTNIGRRFSLLNGRSPDYPISPQTTTPGAQMVSPGLPRNPSALDFKTEPAGRVSSNPGPTGLGSMIHRRLSSHLVPQAKPSIVPLLDETTHSPRPATSSVSLDKFKLSSEQQSISGSFRRRKSIEAEMEGRRRSHSNASSISSKLARLLRTGSQRSHPRVLEKESFTGSDTEDLAAEPSVGSSSPADALGRMSLDGSLPRQSLEHIETGSRSSSQGYIPSPDRPLPAGWESRFRTNAPRRGSNLSEEFTNRVAEEEIDWDGSISDEDDYDDETSQSVAAPTPVAGMNPLWRRARNDNLGVDAQSSAPIVSAAPSLEPIPDGSPSAPTTFPLRSPSRPTLPEIDTGSSDPLFRTSSRTSSRPSHSPFPNSFVERARSPFAGYHDDTRNSPRRKGLNWQTGAPGLADNDEDEGLAISFGSKRGRRGSVQKPTMSEDK
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 10757
B YPD 30°C, concentration: 5x10^7 cells/ml 8795
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 9822
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 5137
E YPD 37°C, concentration: 5x10^7 cells/ml 5486
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 8484
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03791 5' flanking region primer 1GAAGCATCCTCAAAAGGG
L2CNAG_03791 5' flanking region primer 2TCACTGGCCGTCGTTTTACTGGCTGGAGATTTGAAAGAG
R1CNAG_03791 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGCTTTTGGAAGTAAACGGGG
R2CNAG_03791 3' flanking region primer 2GCAACTCGTCAAAGACCTG
SOCNAG_03791 diagnostic screening primer, pairing with B79CGACTTCTTCAGCAATGG
POCNAG_03791 Southern blot probe primerTATTCCAGTCCGAGTAGCG
STMNAT#210 STM primerCTAGAGCCCGCCACAACGCT
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG