Detailed Locus Information for CNAG_03358T0
| Species | Cryptococcus neoformans var. grubii H99 | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Annotation | phosphoglycerate kinase | ||||||||||||||||||
| Gene Symbol | Unknown | ||||||||||||||||||
| Kinase Superfamily | Phosphoglycerate kinase | ||||||||||||||||||
| Kinase Family | Phosphoglycerate kinase | ||||||||||||||||||
| KEGG Linkout | KEGG::CNG02220 | ||||||||||||||||||
| Genome Browser | JBrowse link goes here. Sequence information also available from JBrowse. | ||||||||||||||||||
| Kinase Prediction | N/A | ||||||||||||||||||
| Functional Annotation |
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| Amino Acid Sequence | MSLSSKLNITDVDLKGERVLIRVDFNVPMDKEGKITNPARIVAALPTIKYAIDNGAKSVILMSHLGRPDGSPNPKYSLKPVASKLSELLSKDVKFLPECVGDEVKSEVLKGENGQVFLLENLRFHIEEEGKGKKGDEKVKADPEAVKKFREQLTELGTVYINDAFGTAHRAHSSMVGVQLPKRAAGFLMKKELEYFAKVLEKPERPFLAILGGAKVADKIQLIENMLDQVNTLIICGGMSFTFKKTLNNVEIGTSLFDEAGSKQVKDLVEKAKKNNVKLVFPVDYVTADKFDKDAKTGSATDESGIPSDWMGLDCGPKSRELFAQTVAEAKTILWNGPAGVFEFPAFAGGSNALLEACIKAAKNGSTVIVGGGDTATLVAQAGKEDELSHVSTGGGASLELLEGKTLPGVAELSEKK | ||||||||||||||||||
Expression Data
| Notation | Condition | Normalized Raw Expression |
|---|---|---|
| A | YPD 30°C, concentration: 2x 10^8 cells/ml | 45703 |
| B | YPD 30°C, concentration: 5x10^7 cells/ml | 56359 |
| C | YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml | 56148 |
| D | YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml | 59950 |
| E | YPD 37°C, concentration: 5x10^7 cells/ml | 44821 |
| F | YP+galactose 30°C, concentration: 2x10^8 cells/ml | 43066 |
Gene Disruption Strategy
| Primer Name | Description | Primer Sequence |
|---|---|---|
| L1 | CNAG_03358 5' flanking region primer 1 | GCAGAATCGTGAAACATTACCC |
| L2 | CNAG_03358 5' flanking region primer 2 | TCACTGGCCGTCGTTTTACTCATTGAGGAGGTAGGGAGG |
| R1 | CNAG_03358 3' flanking region primer 1 | CATGGTCATAGCTGTTTCCTGTGAAAGGTGTCGGGGATAG |
| R2 | CNAG_03358 3' flanking region primer 2 | ACGGAGAAGCAGGAACATC |
| SO | CNAG_03358 diagnostic screening primer, pairing with B79 | CAGACAATCGCAGAGTGAG |
| PO | CNAG_03358 Southern blot probe primer | CTCTCGGAACTTCTTGACG |
| STM | NAT#230 STM primer | ATGTAGGTAGGGTGATAGGT |
| STM common | STM common primer | GCATGCCCTGCCCCTAAGAATTCG |