Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03355T0
SpeciesCryptococcus neoformans var. grubii H99
Gene Annotationhypothetical protein
Gene SymbolTCO4
Kinase SuperfamilyHomodimeric domain of signal transducing histidine kinase
Kinase FamilyHomodimeric domain of signal transducing histidine kinase
KEGG Linkout KEGG::CNG02240 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction N/A
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K20974 KEGG 2.9e-187 338.8
K20974 KEGG 2.9e-187 282
Pfam Annotation
Pfam AccessionE-valueScore
PF00072 Pfam 7.7e-45 92.9
PF02518 Pfam 2.2e-47 78.5
PF02518 Pfam 2.2e-47 77.3
Amino Acid SequenceMLGNGSIDYSALPTRYLESYPYPSLVLLVPIPPPSQPSQEELDEFPSPSFNTEAPKLFTCADHGTSQPFDIVWANEKWRNLTNDQPLLNCLNVKGARKLGNWIGGVKEATGNGKEQIVHVAKAAGVGIPEVLAKMISSQSSSEFSVTQMLHSADPGQDRMPQPPEHFWAPEHPYEDRSTKSDHLSNSSELTGIEVEEGMQNNPATVIIELVNPGTVTLEFTKTIMPVTRLGTGGLKAPMNTHSFAIITTVPKSLFVPNSSFQSSPLASPELQGVQDEAATRSNYPLAIPKTTILSPSQSPPPLPFSTAPQSSLTPNPDTDNISPAKKSLIDELKGPGILPPSKKHSAPLMFNGDGTVSRQAQGLEARGESRTSVDVLMDQTDWSKTPLGPLEQWSQSLKTIVGLVLNYPHQCCLWWGEELTLIYNEPYARAIHKHPDIFGMSGPVAWSEIWNSIGPLSEVVLSGTPVWKEDDFLLFKQLPHQGEGVWEEYHTWMWVPVLQEDGTFGGLWNATVRTTKKVLAERRTATVREMGQRTSIARTMDEFNDAVCEILESNPRDTPFAALYRVEPTLSSKGKGLVANTAEITKNDPNEVQVDVTLVGRVGIPESHPSTPSSMSFTIRQRRGSSINRFPQGLNSSPAMSLISSTGGISHIPLGDIDEGESEPYPTPELKHWPIREALQCKRLILVENVAGLIKDYPIRVWDELPNAAIVVPIRSDSEEGIPSAVLVLGLNIRRPFDEDYESFIHVLRLQLASGIAAVRSYEAERLRLDELAALDRAKSLLFSNVSHELRTPLTLISGPIEDLLMETKDGPKKEMLLMARRNLRRLSRLVSTLMDVSRLEAGRLRGTFRPVNLGIMTRDLALLFKATIEKAKLKYIIEVDTSPENVFVDPEHWEKIVFNLIGNAMKYTMTGFVKIELYYSSGQAILTVQDSGVGIPRSDIHLIGERFHRVQSVSRSHEGTGIGLSLTKELISLHGGTLEIESFTEEESIDGSHGSTFRVKIPIGSDHLPFEAIDTGRMPNMPQLTYGQGLVDEAMQWVRDRETGSVVSSDESSSALTGDSSGMGNAKPIDPNTLYFQKEDVIMLVEDSADTRRYMKSIFSQYCQVVEARDGQEALELCQKSVPDLIISDVMMPHLNGFELLVALKRSKDLKMVPVIMLTARGADESKVDGIMAGAEDYLAKPFSAREIVARAHMQLQLGKKRRHLEEAFEERTAELRALAEFSPVGIFRADQNGNITFANSAWYKVSGYPMNEPIVDWVDVIIAPHEREAARAFWKEALTSPEKSLMGDWEYANGRWITVTIIRLDAISNGSVGLKGLLGCVTDITERKVHEESQRRQVIEAEQRRLDAEEAKRQQELLIDITSHEIRNPISSVMQCSSLVKTNLLSLQEQLHIAMANHTAFIPTRQLMNNIEEDLEALESIYQCGLTQERISNDVLSLGRIQLDMLQMFDVEFDLSKEAQNIISIFQNEARMKRISLSLKTGDNNKKLGIRYVKADLVRLNQITTNLLSNAIRFTATSSIRHIEVRTDISFDPPEEGTCAVAKDVLSQPPVLKDDMPIFVFLEVADTGPGLSEDECQKLFQRFSQVSPKTHTIFGGSGLGLFVCRKLAELMGGSIEVVSEKGKGSTFRFYIKAKTCRTSPATDSDLRAKMKAQRDTARYFGLRRRPHVLIVEDNLINQTVLARQLKHCNLTCDVANDGLEALEKIRKVSSIENESDGQAFDCVLMDLEMPVMDGLTAVGHIREEENAGKLKKNLVIALTGNARQGQIDLAKASGMDEVVIKPYRLDDLLHKIEEVIKIRVAEEDEEINQEVEVETKAVEQARKIATPMESATDKS
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 54815
B YPD 30°C, concentration: 5x10^7 cells/ml 29254
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 28962
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 14435
E YPD 37°C, concentration: 5x10^7 cells/ml 12103
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 23371
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03355 5' flanking region primer 1AATGCCATAGGACACCTCTGACCC
L2CNAG_03355 5' flanking region primer 2CTGGCCGTCGTTTTACTGTGACTATGGTAAGCACCG
R1CNAG_03355 3' flanking region primer 1GTCATAGCTGTTTCCTGAATGCCATAGGACACCTCTGACCC
R2CNAG_03355 3' flanking region primer 2TGTGACTATGGTAAGCACCG
SOCNAG_03355 diagnostic screening primer, pairing with B79GTTGCTTGGTTTTTCTTCGG
PO1CNAG_03355 Southern blot probe primer 1AAACGGCAGCATTGACTAC
PO2CNAG_03355 Southern blot probe primer 2TATGTAAGCAGCCTGTTCG
STMNAT#123 STM primerCTATCGACCAACCAACACAG
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_03355T0
Mating Data
Growth phenotype for CNAG_03355T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_03355T0
Urease
Urease phenotype for CNAG_03355T0
Melanin
Melanin phenotype for CNAG_03355T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_03355T0
Oxidative Stress
Oxidative stress phenotype for CNAG_03355T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_03355T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_03355T0
ER Stress
ER stress phenotype for CNAG_03355T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_03355T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_03355T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_03355T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_03355T0