Kinase Browser

Detailed locus information, expression profile, gene disruption strategy, and phenotype assay data.

Detailed Locus Information for CNAG_03184T0
SpeciesCryptococcus neoformans var. grubii H99
Gene AnnotationBUB protein kinase
Gene SymbolBUB1
Kinase SuperfamilyProtein kinase-like (PK-like)
Kinase FamilyProtein kinases, catalytic subunit
KEGG Linkout KEGG::CNG03850 
Genome Browser JBrowse link goes here. Sequence information also available from JBrowse.
Kinase Prediction
Microbial Kinome Result
PredictionScoreE-value
Bub510.64.3e-153
Functional Annotation
KEGG Annotation
KO AccessionE-valueScore
K02178 KEGG 1.4e-208 694
Pfam Annotation
Pfam AccessionE-valueScore
PF08311 Pfam 1.2e-33 113.4
Amino Acid SequenceMDSPGVAPTTDFALIESQKENIRPLATGRSAATLSTVFKEPSAADKLLQQGHEQHRKAIQEADRRDAADEDMPDGLSDVLDVYNQYILFTVQHHPSSDAHLLPLLETTTRRFINDARYTHDIRYLKLWIMYARQIDRREEIWAFLESRDIGTRHSLFYEEWATTLEGMGRRKKADEIYRLGIARKASPLDRLKNRHKQFLERIMAPPSGVIPDDDPASAPARTPSRSVLGQVRTAPSSSVSGATQLAPSLRSTSVNNGRKMEIFADEEGKADDTPASEWPEFGSRDERKKENTVEAGPWKGETLPQSAVRGRVAPRTPKVEVFQDGEEGVAPVQTNFEVFSRHKQPPTEAELLKSDPLRHYDTTNLSTDIPSLPAPPSARKPPKPLKSNSSASASTKPSSNVNAVRHVMQPWTCPTDGPLIKLANGTKERRMFNWELVYKDGEEWSFEEIRAKQRGLYGKEWRGEVQEWERQWHKPGSSTPPKPKPLEQCKPPSPTVNTKLAEAEVMRMFDQTIHGGKIGDSDSESDSEDDDEEEDDPIQVAPTPLPARTGSIQMLAPTPGGFVPPTPTPSAASRLFSNNENTKPVVFSDENAVPPSATKKKFNIFSDDPENNPTKTPLGAAPPSATQPRAFGIFSDENQTPAKPISFSTQETPGQRQPLGQSSIFGTPAPVVPGRGALLSRVTEVIEEDAEEDAAAAEEQASRQKEIVEPRVEQQEAQDDVREITQDVEHVTLNDVQEEDELEDAAPRGMRRFNINLMTPITERTCEYTTFTQGTTAGKESFAREEEEEEEEEEKEKVKEEPPISTTSPEDDTTDAGLASGDVDSGFQLPDGFTIHRAEDRLDVHTMVLIDDEGKNEPTGTMTINQDKSVYHTAPAGSLSLSDLPNPCDPISEQTISALLSAIDPPLSSLPGLIDNRTITSNKLDSLEKHTRSKMRRASTSSRTSTSAAGDDTCLLELAGKPYEVRDKLGEGGFGVVFLGIDVDVRRAQDEADSDDEDEEPVDRSLVALKVERPGAMWEAAVLDRIHRRVNANLRASIILPRNLYLFKDESFLILDYCSQGTLLDVVNKSTAIFGGTGQGVDELLAMFFTIELLKLLEGLHSAQFIHGDLKIDNCLVRISDLPNSQWSASYSRTGSNGWSAQGLRLIDFGRAIDLSLFPAGEQQMFKVSHKVDERDCTEMRRGEAWNFQTDYFGLASVAYCLLFGKYMKTEIVGGKIKIDQPLKRYWQTPLWTVFFETLLNPDDQLPITNRLGEIRSDFEGWLEENSQKGGKSLKSLLRKIELAATTSRKL
Expression Data
Notation Condition Normalized Raw Expression
A YPD 30°C, concentration: 2x 10^8 cells/ml 711
B YPD 30°C, concentration: 5x10^7 cells/ml 3118
C YPD 30°C, fluconazole treatment, concentration: 5x10^7 cells/ml 2915
D YPD 30°C, SDS 0.01% treatment, concentration: 5x10^7 cells/ml 3707
E YPD 37°C, concentration: 5x10^7 cells/ml 4816
F YP+galactose 30°C, concentration: 2x10^8 cells/ml 1005
Gene Disruption Strategy
Mutant construction design
Primer NameDescriptionPrimer Sequence
L1CNAG_03184 5' flanking region primer 1GGCAGAGCAGTATTTTTGG
L2CNAG_03184 5' flanking region primer 2TCACTGGCCGTCGTTTTACAGGTTTTCCTGCGAGTTC
R1CNAG_03184 3' flanking region primer 1CATGGTCATAGCTGTTTCCTGAAGCGACTTTGAGGGATGGC
R2CNAG_03184 3' flanking region primer 2ATCCCAGAACAGTGGCAGAC
SOCNAG_03184 diagnostic screening primer, pairing with B79CAACGAAAACACCAAACCTG
POCNAG_03184 Southern blot probe primerCATTGCGAGTTTGGTAGG
STMNAT#201 STM primerCACCCTCTATCTCGAGAAAGCTCC
STM commonSTM common primerGCATGCCCTGCCCCTAAGAATTCG
Phenotype Assays
Growth
Growth phenotype for CNAG_03184T0
Mating Data
Growth phenotype for CNAG_03184T0
Virulence Factor Production
Capsule
Capsule phenotype for CNAG_03184T0
Urease
Urease phenotype for CNAG_03184T0
Melanin
Melanin phenotype for CNAG_03184T0
Stress Response and Adaptation
Osmotic Stress
Osmotic stress phenotype for CNAG_03184T0
Oxidative Stress
Oxidative stress phenotype for CNAG_03184T0
Heavy Metal Stress
Heavy metal stress phenotype for CNAG_03184T0
Cell Wall Stress
Cell wall stress phenotype for CNAG_03184T0
ER Stress
ER stress phenotype for CNAG_03184T0
Genotoxic Stress
Genotoxic stress phenotype for CNAG_03184T0
Antifungal Drug Susceptibility
Antifungal drug susceptibility for CNAG_03184T0
Virulence Data by the Insect Host Model (Galleria mellonella)
Galleria mellonella virulence data for CNAG_03184T0
Lung Infectivity Data by the Signature-tag Mutagenesis-based Murine Host Model
STM murine host model data for CNAG_03184T0